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Article
Nature Structural & Molecular Biology 13, 209 - 217 (2006)
Published online: 19 February 2006; | doi:10.1038/nsmb1056

Close membrane-membrane proximity induced by Ca2+-dependent multivalent binding of synaptotagmin-1 to phospholipids

Demet Araç, Xiaocheng Chen, Htet A Khant, Josep Ubach, Steven J Ludtke, Masahide Kikkawa, Arthur E Johnson, Wah Chiu, Thomas C Südhof & Josep Rizo

Supplementary Fig. 1 (pdf 691K)
Problems with labeling C277 of synaptotagmin 1

Supplementary Fig. 2 (pdf 619K)
Reconstruction of the oligomeric model of synaptotagmin 1

Supplementary Fig. 3 (pdf 1136K)
Negative stain em analysis of synpatotagmin/calcium/lipid samples

Supplementary Fig. 4 (pdf 227K)
Synaptotagmin does not oligomerize

Supplementary Fig. 5 (pdf 78K)
Calcium dependence of synaptotagmin induced vesicle clustering

Supplementary Fig. 6 (pdf 257K)
Diverse considerations supporting the model of Figure 6

Supplementary Video 1 (mpg 2.2M)
Tomographic 3D reconstruction of a vesicle cluster

Supplementary Methods (pdf 17K)


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Nature Structural & Molecular Biology
ISSN: 1545-9993
EISSN: 1545-9985
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