Nature Structural & Molecular Biology
12, 32 - 37 (2004)
Published online: 26 December 2004; | doi:10.1038/nsmb880
Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitmentClemens Steegborn, Tatiana N Litvin, Lonny R Levin, Jochen Buck
& Hao WuSupplementary Fig. 1 (pdf 42K)
Kinetics of purified cyanobacterial sAC (CyaC). Supplementary Fig. 2 (pdf 340K)
Model of ATP−calcium bound to the sAC active site. Supplementary Fig. 3 (pdf 98K)
Bicarbonate activation of sAC at various pH values. Supplementary Fig. 4 (pdf 226K)
Active site of the sAC−Rp-ATP S complex. Supplementary Fig. 5 (pdf 26K)
Stereospecific inhibition of sAC by ATPS. Supplementary Table 1 (pdf 20K)
Effect of pH changes and anions on sAC activity and on sAC crystals. Supplementary Video 1 (mpg 1,055K)
Simulated transition between the open and closed form of sAC−, -Me-ATP. Supplementary Video 2 (mpg 2,927K)
Model for catalysis by Class III nucleotidyl cyclases. Supplementary Methods (pdf 20K)
Estimation of the off-rate for the sAC−bicarbonate complex.
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