 | Figure 1
Nature Structural & Molecular Biology
11, 1107 - 1113 (2004)
Published online: 10 October 2004; | doi:10.1038/nsmb842
Observation of internal cleavage and ligation reactions of a ribozymeMichelle K Nahas, Timothy J Wilson, Sungchul Hohng, Kaera Jarvie, David M J Lilley
& Taekjip Ha | | | | Figure 1. Analysis of cleavage and ligation in the hairpin ribozyme.
The ribozyme is constructed by the hybridization of four strands labeled a−d, where the non-blue strand is the substrate. The four arms are labeled A−D. In species 1 the short substrate (green) generates a 3-bp terminal helix in arm A. A cleavage reaction creates species 2 after dissociation of the short 3' product strand, and results in a 5' cleavage product that is terminated by a cyclic phosphate, indicated by the red triangle. Species 3 is generated by annealing a 3' product (magenta) that forms a 7-bp terminal helix in arm A. A ligation reaction generates species 4, whereupon cycles of cleavage and ligation are possible.
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