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Double duty

Nature Structural & Molecular Biology volume 11, pages 910–911 (2004)Cite this article

A second high-affinity binding site for the I-TevI homing endonuclease has been discovered. Surprisingly, the DNA sequence recognized is the protein's own operator; at this site, the endonuclease represses its own transcription instead of cleaving the DNA and inducing intron homing.

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Figure 1: Comparison of homing and transcriptional autorepression by the I-TevI DNA endonuclease.

References

  1. Quirk, S.M., Bell-Pedersen, D. & Belfort, M. Cell 56, 455–465 (1989).

    Article  CAS  PubMed  Google Scholar 

  2. Belfort, M. & Roberts, R.J. Nucleic Acids Res. 25, 3379–3388 (1997).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Belfort, M., Derbyshire, V., Cousineau, B. & Lambowitz, A.M. In Mobile DNA II (eds. Craig, N., Craigie, R., Gellert, M. & Lambowitz, A.M.), 761–783 (ASM Press, Washington, DC, 2002).

    Book  Google Scholar 

  4. Edgell, D.R. et al. Nat. Struct. Mol. Biol. 11, 936–944 (2004).

    Article  CAS  PubMed  Google Scholar 

  5. Bryk, M. et al. EMBO J. 12, 4040–4041 (1993).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Mueller, J.E., Clyman, J., Huang, Y.J., Parker, M.M. & Belfort, M. Genes Dev. 10, 351–364 (1996).

    Article  CAS  PubMed  Google Scholar 

  7. Belfort, M. Trends Genet. 5, 209–213 (1989).

    Article  CAS  PubMed  Google Scholar 

  8. Chatterjee, P., Brady, K.L., Solem, A., Ho, Y. & Caprara, M.G. J. Mol. Biol. 329, 239–251 (2003).

    Article  CAS  PubMed  Google Scholar 

  9. Geese, W.J., Kwon, Y.K., Wen, X. & Waring, R.B. Eur. J. Biochem. 270, 1543–1554 (2003).

    Article  CAS  PubMed  Google Scholar 

  10. Bolduc, J.M. et al. Genes Dev. 17, 2875–2888 (2003).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Belfort, M. Genes Dev. 17, 2860–2863 (2003).

    Article  CAS  PubMed  Google Scholar 

  12. Van Roey, P., Waddling, C.A., Fox, K.M., Belfort, M. & Derbyshire, V. EMBO J. 20, 3631–3637 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Dean, A.B. et al. Proc. Natl. Acad. Sci. USA 99, 8554–8561 (2002).

    Article  CAS  PubMed  Google Scholar 

  14. Bechhofer, D.H., Hue, K.K. & Shub, D.A. Proc. Natl. Acad. Sci. USA 91, 11669–11673 (1994).

    Article  CAS  PubMed  Google Scholar 

  15. Edgell, D.R. & Shub, D.A. Proc. Natl. Acad. Sci. USA 98, 7898–7903 (2001).

    Article  CAS  PubMed  Google Scholar 

  16. Gott, J.M. et al. Genes Dev. 2, 1791–1799 (1988).

    Article  CAS  PubMed  Google Scholar 

  17. Loizos, N., Tillier, E.R. & Belfort, M. Proc. Natl. Acad. Sci. USA 91, 11983–11987 (1994).

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank T. Skern for comments on the manuscript. Preparation of this News & Views was supported by the Austrian Science Foundation, FWF grant no. Z72.

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  1. University Departments of the Vienna Biocenter, Department of Microbiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/4, Vienna, A-1030, Austria

    Nikolai Windbichler & Renée Schroeder

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  1. Nikolai Windbichler
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  2. Renée Schroeder
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Windbichler, N., Schroeder, R. Double duty. Nat Struct Mol Biol 11, 910–911 (2004). https://doi.org/10.1038/nsmb1004-910

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