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Snapshots of transition states?

Nature Structural Biology volume 10, pages 75–77 (2003)Cite this article

Time-resolved X-ray absorption has been used to investigate the structure of the Zn active site in alcohol dehydrogenase during catalysis. The results support a new mechanistic model for catalysis.

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Figure 1: Schematic illustration of rapid-mixing device used for time-resolved spectroscopic measurements.
Figure 2: Geometry for a, dispersive and b, single-wavelength XAS measaurements.

References

  1. Kleifeld, O., Frenkel, A., Martin, J.M.L. & Sagi, I. Nat. Struct. Biol. 10, 98–103 (2003).

    Article  CAS  Google Scholar 

  2. Bertini, I., Luchinat, C., Maret, W. & Zeppezuer, M. Zinc Enzymes, (Birkhäuser, Boston, 1986).

    Google Scholar 

  3. Eklund, H., Jones, A. & Schneider, G. in Zinc Enzymes (eds. Bertini, I., Luchinat, C., Maret, W. & Zeppezuer, M.) 377–392 (Birkhäuser, Boston, 1986).

    Google Scholar 

  4. Eklund, H. & Brändén, C.I. in Biological Macromolecules and Assemblies (eds. Jurnak, F.A. & McPherson, A.) 73–143 (John Wiley & Sons, New York, 1987).

    Google Scholar 

  5. Kimblin, C., Bridgewater, B.M., Churchill, D.G., Hascall, T. & Parkin, G. Inorg. Chem. 39, 4240–4243 (2000).

    Article  CAS  Google Scholar 

  6. Meijers, R. et al. J. Biol. Chem. 276, 9316–9321 (2001).

    Article  CAS  Google Scholar 

  7. Penner-Hahn, J.E. Coord. Chem. Rev. 192, 1101–1123 (1999).

    Article  Google Scholar 

  8. Clark-Baldwin, K. et al. J. Am. Chem. Soc. 120, 8403–8409 (1998).

    Article  Google Scholar 

  9. Kleifeld, O. et al. Biochemistry 39, 7702–7711 (2000).

    Article  CAS  Google Scholar 

  10. Zhang, K., Dong, J. & Auld, D.S. Physica B 209, 719–721 (1995).

    Article  Google Scholar 

  11. Richwin, M., Zaeper, R., Lutzenkirchen-Hecht, D. & Frahm, R. Rev. Sci. Instrum. 73, 1668–1670 (2002).

    Article  CAS  Google Scholar 

  12. Haumann, M., Grabolle, M., Neisius, T. & Dau, H. FEBS Lett. 512, 116–120 (2002).

    Article  CAS  Google Scholar 

  13. Hwang, J. et al. Science 287, 122–125 (2000).

    Article  CAS  Google Scholar 

  14. Riggs-Gelasco, P.J. et al. J. Am. Chem. Soc. 120, 849–860 (1998).

    Article  CAS  Google Scholar 

  15. Frenkel, A., Kleifeld, O., Wasserman, S.R. & Sagi, I. J. Chem. Phys. 116, 9449–9456 (2002).

    Article  CAS  Google Scholar 

Download references

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  1. Department of Chemistry and the Biophysics Research Division, The University of Michigan, Ann Arbor, 48109-1055, Michigan, USA

    James E. Penner-Hahn

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  1. James E. Penner-Hahn
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Penner-Hahn, J. Snapshots of transition states?. Nat Struct Mol Biol 10, 75–77 (2003). https://doi.org/10.1038/nsb0203-75

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