Nature Structural Biology
10, 899 - 906 (2003)
Published online: 19 October 2003; | doi:10.1038/nsb1003
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopyMikel Valle, Andrey Zavialov, Wen Li, Scott M Stagg, Jayati Sengupta, Rikke C Nielsen, Poul Nissen, Stephen C Harvey, Måns Ehrenberg
& Joachim FrankSupplementary Fig. 1 (pdf 1.5M) Supplementary Video 1. (mov 2.8M)
Accommodation process on the 70S ribosome.By alternating
between cryoEM maps of (i)the 70S ribosome with the stalled ternary complex
(70S  fMet-tRNA
fMet
Phe-tRNA
Phe
EF-Tu GDP kir)and (ii)the complex bearing the
accommodated A-site tRNA (70S tRNA
fMet
MP-tRNA
Phe
),the dynamic transition during
the incorporation of the aa-tRNA can be observed.In the rendering,the segmented and
differently colored volumes allow the ribosomal subunits (large subunit in blue,small
subunit in yellow)and the tRNAs in P (green)and E sites (orange)to be distinguished.
The A/T-and A-site tRNAs are depicted in the same color (magenta),so that the
trajectory of the tRNA can be more easily followed during the accommodation. Supplementary Video 2. (mov 3.6M)
tRNA movement and conformational change during
accommodation.
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