A three−dimensional model of the Rev−binding element of HIV−1 derived from analyses of aptamers

Coordinated variations in the sequence of the Rev−binding element of HIV−1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three−dimensional models of the wild−type Rev−binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non−Watson−Crick pairings open the major groove allowing access of an −helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.

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