FIGURE 3 Immunohistochemical analysis of synovial tissue from the biopsy specimen of the right wrist.
From the following article:
A case of human T lymphotropic virus type I-associated synovial swelling
Gregory Dennis and Puja Chitkara
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Figure 3. Immunohistochemical analysis of synovial tissue from the biopsy specimen of the right wrist.
(A) Histological section of formalin-fixed, paraffin-embedded synovial tissue biopsy. A dense lymphocytic infiltrate composed of cells displaying nuclear atypia and mild pleomorphism is seen in the synovial tissue. The morphological appearance of this infiltrate is very suggestive of ATLL involvement. (Hematoxylin and eosin stain, 
40.) (B) Immunohistochemical stain for CD3 shows the atypical lymphoid cells to be strongly positive for this T-cell marker. (CD3 immunohistochemical stain, 40.) (C) The CD3+ T cells are predominantly and diffusely positive for CD4 by immunohistochemistry. (CD4 immunohistochemical stain, 40.) (D) Immunohistochemical stain reveals scattered CD8+ T cells, which are probably reactive. (CD8 immunohistochmical stain, 40.) (E) CD25 immunohistochemical stain reveals diffuse and strong positivity in the atypical cells. This finding supports the diagnosis of ATLL. (CD25 immunohistochemical stain, 40.) (F) CD30+ atypical cells showing cytoplasmic and membranous staining pattern. (CD30 immunohistochemical stain, 60.) Abbreviation: ATLL, adult T-cell lymphoma/leukemia.
