New technologies: methods and applications

The study of microbiology is undergoing a renaissance owing to the development and application of a diverse range of new technologies. A little more than a decade ago, the visualization of fundamental subcellular structures and processes was almost impossible; now, following the successful marriage of biophysics and microbiology, the number of single-molecule studies in living cells is growing at a rapid pace. Increasingly sophisticated sequencing approaches, coupled with improved analytical and computational tools, allow us to rapidly indentify pathogens, track infectious disease outbreaks and identify links between microorganisms and disease. Furthermore, innovative micro-scale engineering has facilitated the simulation of microbial environments with remarkable precision and has opened the door to understanding microbial heterogeneity at an unprecedented level of detail. In this series of articles, Nature Reviews Microbiology explores some of the most recent technological developments and their applications, highlighting the ways in which this powerful toolkit is changing the face of modern-day microbiology.




September Vol 15 No 9

Engineering of obligate intracellular bacteria: progress, challenges and paradigms

Erin E. McClure, Adela S. Oliva Chávez, Dana K. Shaw, Jason A. Carlyon, Roman R. Ganta, Susan M. Noh, David O. Wood, Patrik M. Bavoil, Kelly A. Brayton, Juan J. Martinez, Jere W. McBride, Raphael H. Valdivia, Ulrike G. Munderloh & Joao H. F. Pedra

June Vol 15 No 6

A CRISPR toolbox to study virus–host interactions

Andreas S. Puschnik, Karim Majzoub, Yaw Shin Ooi & Jan E. Carette

March Vol 15 No 3

Clinical and biological insights from viral genome sequencing

Charlotte J. Houldcroft, Mathew A. Beale & Judith Breuer



July Vol 14 No 7

Advancing microbial sciences by individual-based modelling

Ferdi L. Hellweger, Robert J. Clegg, James R. Clark, Caroline M. Plugge & Jan-Ulrich Kreft

April Vol 14 No 4

A new view into prokaryotic cell biology from electron cryotomography

Catherine M. Oikonomou & Grant J. Jensen

February Vol 14 No 2

The design and analysis of transposon insertion sequencing experiments

Michael C. Chao, Sören Abel, Brigid M. Davis & Matthew K. Waldor



December 2015 Vol 13 No 12

Live from under the lens: exploring microbial motility with dynamic imaging and microfluidics

Kwangmin Son, Douglas R. Brumley & Roman Stocker

December 2015 Vol 13 No 12

Twenty years of bacterial genome sequencing

Nicholas J. Loman & Mark J. Pallen

September 2015 Vol 13 No 9

Bacterial protein networks: properties and functions

Athanasios Typas & Victor Sourjik

June 2015 Vol 13 No 6

Advances in molecular genetic systems in malaria

Tania F. de Koning-Ward, Paul R. Gilson & Brendan S. Crabb

March 2015 Vol 13 No 3

Rising to the challenge: accelerated pace of discovery transforms marine virology

Jennifer R. Brum & Matthew B. Sullivan



August 2014 Vol 12 No 8

Adding new dimensions: towards an integrative understanding of HIV-1 spread

Oliver T. Fackler, Thomas T. Murooka, Andrea Imle & Thorsten R. Mempel

January 2014 Vol 12 No 1

Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging

Andreas Gahlmann & W. E. Moerner



July 2013 Vol 11 No 7

Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms

Tim van Opijnen & Andrew Camilli

May 2013 Vol 11 No 5

Bacterial replication, transcription and translation: mechanistic insights from single-molecule biochemical studies

Andrew Robinson & Antoine M. van Oijen

Going local: technologies for exploring bacterial microenvironments

Aimee K. Wessel1, Laura Hmelo, Matthew R. Parsek & Marvin Whiteley

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