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Nature Reviews Microbiology 6, 260–261 (1 April 2008) | doi:10.1038/nrmicro1881
Genomic 'valleys of death'
Abstract
Random fragments are cloned into plasmid vectors before being introduced into Escherichia coli by transformation to be replicated in preparation for DNA sequencing. Although the cloning process is essentially random, the resulting assembled sequences have an uneven distribution across the sequenced genome, with some regions having no clone coverage (Fig.
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