FIGURE 2 | Network reconstruction using ultra-high-accuracy mass spectrometry.

If metabolite masses in a cellular sample are measured comprehensively and with high accuracy, the difference between masses can be used to identify compounds that are related by common metabolic transformations. This information can be used to infer a putative metabolic network. The reconstruction will contain spurious edges, for example, the red and blue metabolites are not directly connected by an enzymatic transformation in the real network (top). Such reactions can be pruned from the reconstruction using measured C¹³ stable-isotope-flux measurements in combination with optimal metabolic network identification analysis, or by correlation measurements. Genetical genomics experiments can lead to further refinement and help to link the inferred reactions to the catalysing enzymes. r, reaction.

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