The foodborne enteropathogen Yersinia pseudotuberculosis uses transcriptional and post-transcriptional thermoregulation to respond to the increase in temperature that occurs during infection of warm-blooded hosts. Previously, one Y. pseudotuberculosis transcript had been shown to be thermoregulated by the presence of an 'RNA thermometer' (RNAT) upstream of the coding region. RNATs regulate translation by altering the accessibility of the ribosome-binding site to the ribosome, based on temperature-dependent changes to the RNA structure. Righetti et al. took advantage of recent methodological advances to structurally probe the Y. pseudotuberculosis transcriptome at environmental (25 °C), host (37 °C) and heat-shock (42 °C) temperatures. Twenty candidate RNATs were validated using a heat-stable reporter protein, and these included RNATs that regulated genes that are involved in oxidative stress or virulence, including the adhesin gene ailA.