Host-cell entry and exit are traditionally viewed as processes that viruses undergo as independent units. However, Chen et al. now describe a novel intercellular transport pathway, in which clusters of approximately 20 mature enterovirus particles, such as poliovirus, are packaged into phosphatidylserine (PS)-enriched vesicles and are transmitted in unison between cells in a non-lytic manner. Compared with free virus particles, transmission via vesicles led to a substantial increase in infection efficiency, even though in both cases cell entry was dependent on the receptor CD155. The increased infection efficiency might be due to interactions between the vesicles and host cell PS receptors, as vesicles in which PS was masked were not infectious. Aside from casting doubt on the paradigm that viruses enter and exit cells as independent units, these findings suggest that genetic cooperation might occur between individual enterovirus genomes.