Stem cell states: naive to primed pluripotency

Leehee Weinberger, Yair S. Manor and Jacob H. Hanna

Pluripotency refers to the ability of cells to differentiate into cell types of all three embryonic germ layers. Deriving and maintaining pluripotent stem cells could thus enable the generation of valuable cells for tissue replacement therapies and for the study of mammalian development. Embryonic cells are pluripotent for only a short window of time in the embryo: they become pluripotent within the inner cell mass of pre-implantation embryos and gradually lose potency as they commit to differentiating into specific somatic lineages early during post-implantation development. In pre-implantation embryos, pluripotent stem cells are referred to as 'naive', and they become 'primed' during post-implantation development. Efforts are being made to elucidate the signalling pathways that regulate naive and primed pluripotency, with a view to optimizing culture conditions for the induction or maintenance of these states in vitro.

This Poster provides an overview of the signalling pathways currently known to promote or suppress the naive and primed pluripotency states in mice, and highlights potential differences between mice and humans. It also provides an overview of culture conditions that confer naive and/or primed features to cells.

The Poster is freely available thanks to support from STEMCELL Technologies

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