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Nature Reviews Molecular Cell Biology 7, 449–456 (1 June 2006) | doi:10.1038/nrm1929
Visualization of molecular interactions by fluorescence complementation
Abstract
The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.
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