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Review

Nature Reviews Molecular Cell Biology 7, 177–187 (1 March 2006) | doi:10.1038/nrm1860

Building mammalian signalling pathways with RNAi screens

Jason Moffat & David M. Sabatini

Recent advances in RNA interference (RNAi)-mediated gene-knockdown technologies have opened up the possibility of large-scale functional discovery in mammalian systems. RNAi screening could help us to delineate the architecture of signalling pathways much faster than by using traditional approaches.