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A visual approach to proteomics

Nature Reviews Molecular Cell Biology volume 7, pages 225–230 (2006)Cite this article

Abstract

Cryo-electron tomography is an emerging imaging technique that has unique potential for molecular cell biology. At the present resolution of 4–5 nm, large supramolecular structures can be studied in unperturbed cellular environments and, in the future, it will become possible to map molecular landscapes inside cells in a more comprehensive manner. 'Visual proteomics' aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the macromolecular interactions that underlie cellular functions.

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Figure 1: Principles of electron tomography.
Figure 2: The advantage of tomographic imaging for visualizing the architecture of cryosectioned eukaryotic cells.
Figure 3: From proteomics inventories to a cellular protein atlas.

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Authors and Affiliations

  1. the Department of Structural Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, Martinsried, D-82152, Germany

    Stephan Nickell, Christine Kofler, Andrew P. Leis & Wolfgang Baumeister

Authors
  1. Stephan Nickell
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  2. Christine Kofler
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  3. Andrew P. Leis
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  4. Wolfgang Baumeister
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Corresponding author

Correspondence to Wolfgang Baumeister.

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FURTHER INFORMATION

3D electron microscopy at Utrecht University

Cryo-electron tomography of Dictyostelium discoideum (movie)

High Resolution Electron Microscopy at the National Cancer Institute

Institute for Molecular Bioscience, The University of Queensland, Australia

Max-Planck-Institute for Biochemistry, Department of Molecular Structural Biology

Molecular Art | Molecular Science, Home of David S. Goodsell

Resource for the Visualization of Biological Complexity, Wadsworth Center

The Boulder Laboratory for 3-D Electron Microscopy of Cells

The Jensen Laboratory for Cryo-Electron Microscopy

The Three Dimensional Electron Microscopy (3D-EM) Network of Excellence

University of California San Francisco, Macromolecular Structure Group

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Nickell, S., Kofler, C., Leis, A. et al. A visual approach to proteomics. Nat Rev Mol Cell Biol 7, 225–230 (2006). https://doi.org/10.1038/nrm1861

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