Review

Nature Reviews Molecular Cell Biology 5, 699-711 (September 2004) | doi:10.1038/nrm1468

The abc's (and xyz's) of peptide sequencing

Hanno Steen1 & Matthias Mann2  About the authors

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Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key — but little understood — technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.

Author affiliations

  1. Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

Correspondence to: Matthias Mann2 Email: mann@bmb.sdu.dk

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