Review
Nature Reviews Molecular Cell Biology 3, 906-918 (December 2002) | doi:10.1038/nrm976
Creating new fluorescent probes for cell biology
Jin Zhang1, Robert E. Campbell1, Alice Y. Ting1,2 & Roger Y. Tsien1,3 About the authors
Abstract
Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein–protein interactions.
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Author affiliations
- Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093-0647, USA.
- Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18–496, Cambridge, Massachusetts 02139, USA
- Department of Chemistry & Biochemistry and Howard Hughes Medical Institute, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093–0647, USA.
Correspondence to: Roger Y. Tsien1,3 Email: rtsien@ucsd.edu
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