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Epigenetics

Cytosolic EZH1 muscles PRC2 out of the nucleus

Nature Reviews Molecular Cell Biology volume 18, pages 276–277 (2017)Cite this article

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Differentiated C2C12 mouse skeletal muscle cells (myotubes) express EZH1 but not EZH2. Following atrophic stress, H3K27me3 levels increased in cultured C2C12 myotubes; the increase was dependent on EZH1 expression and was reversed once the culture medium was refreshed. H3K27me3 levels were also examined in mice, in hindlimb muscles that atrophied following ten days of immobilization. Western blotting and immunofluorescence experiments revealed that H3K27me3 levels were increased in atrophic muscles, and decreased to control levels when muscles were allowed to recover for 24 days after disuse.

Chromatin immunoprecipitation followed by sequencing and gene expression analyses revealed a redistribution of H3K27me3 throughout the genome in atrophic conditions, and a doubling of the number of genes that were targeted and repressed by PRC2-EZH1. Notably, many of the downregulated genes were muscle-specific. In particular, in atrophic myotubes, increased H3K27me3 levels in the promoters of two examined genes, and consequently their transcriptional repression, were dependent on EZH1.

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References

  1. Bodega, B. et al. A cytosolic Ezh1 isoform modulates a PRC2–Ezh1 epigenetic adaptive response in postmitotic cells. Nat. Struct. Mol. Biol. http://dx.doi.org/10.1038/nsmb.3392 (2017)

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Authors
  1. Eytan Zlotorynski
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Blackledge, N. P., Rose, N. R. & Klose, R. J. Targeting Polycomb systems to regulate gene expression: modifications to a complex story. Nat. Rev. Mol. Cell Biol. 16, 643–649 (2015)

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Zlotorynski, E. Cytosolic EZH1 muscles PRC2 out of the nucleus. Nat Rev Mol Cell Biol 18, 276–277 (2017). https://doi.org/10.1038/nrm.2017.37

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  • DOI: https://doi.org/10.1038/nrm.2017.37

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