Genomic variable lamina-associated domains (vLADs) bind the nuclear lamina and repress genes in a cell-state-specific manner. Harr et al. developed the tagged chromosomal insertion site (TCIS) system to integrate short (<2.5-kb) DNA fragments into the genome, identified mouse fibroblast-specific vLADs and derived lamina-associated sequences (LASs) from their borders. Several LAS insertions could target chromatin to the lamina by binding the transcriptional repressor YY1. Targeting of both LAS-containing TCISs and endogenous LADs to the lamina required YY1 and lamin C, as well as the facultative heterochromatin marks H3K27me3 and H3K9me2/3.
References
Harr, J. C., et al. Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins. J. Cell Biol. 208, 33–52 (2015)
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Zlotorynski, E. Targeting chromatin to the lamina. Nat Rev Mol Cell Biol 16, 68 (2015). https://doi.org/10.1038/nrm3948
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DOI: https://doi.org/10.1038/nrm3948
