Review

Nature Reviews Molecular Cell Biology 11, 789-801 (November 2010) | doi:10.1038/nrm2973

Generating and navigating proteome maps using mass spectrometry

Christian H. Ahrens1, Erich Brunner1, Ermir Qeli1, Konrad Basler1,2 & Ruedi Aebersold3  About the authors

Top

Proteomes, the ensembles of all proteins expressed by cells or tissues, are typically analysed by mass spectrometry. Recent technical and computational advances have greatly increased the fraction of a proteome that can be identified and quantified in a single study. Current mass spectrometry-based proteomic strategies have the potential to reproducibly, accurately, quantitatively and comprehensively measure any protein or whole proteomes from cells and tissues at different states. Achieving these goals will require complete proteome maps and analytical strategies that use these maps as prior information and will greatly enhance the impact of proteomics on biological and clinical research.

Author affiliations

  1. Quantitative Model Organism Proteomics, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
  2. Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
  3. Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Strasse 16, CH-8093 Zurich, Switzerland, and Faculty of Science, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Correspondence to: Christian H. Ahrens1 Email: christian.ahrens@imls.uzh.ch

Correspondence to: Ruedi Aebersold3 Email: aebersold@imsb.biol.ethz.ch

Published online 14 October 2010