TABLE 1 | Evidence for widespread transcription
From the following article:
Genome-wide transcription and the implications for genomic organization
Philipp Kapranov, Aarron T. Willingham & Thomas R. Gingeras
Nature Reviews Genetics 8, 413-423 (June 2007)
doi:10.1038/nrg2083
| Method | Organism | Refs |
|---|---|---|
| Abundance of polysome-associated polyA RNA | Homo sapiens | 82 |
| Nucleic acid hybridization reassociation kinetics (Cot curves) | Strongylocentrotus purpuratus (sea urchin) | 83 |
| H. sapiens | 84 | |
| Transcription of satellite DNA on chromosomes in oogenesis | Triturus cristatus carnifex (crested newt) | 85 |
| Abundance of 5'-capped RNAs versus those with 3' polyA | Cricetulus griseus (hamster) | 86 |
| Tiling arrays | ||
| Whole genome | Escherichia coli | 87 |
| Arabidopsis thaliana | 88 | |
| Drosophila melanogaster | 15,89 | |
| H. sapiens | 90 | |
| Oryza sativa (rice) | 91 | |
| S. purpuratus | 92 | |
| Whole genome, high resolution | Saccharomyces cerevisiae | 93 |
| H. sapiens | 9 | |
| Ten chromosomes, high resolution | H. sapiens | 3 |
| Chromosome 21–22 | H. sapiens | 94 |
| Chromosome 22 | H. sapiens | 95 |
| Sequencing | ||
| CAGE tags | H. sapiens and Mus musculus | 7 |
| CAGE tags to identify promoters | H. sapiens and M. musculus | 13,96 |
| PET tags | M. musculus | 97 |
| SAGE tags | H. sapiens | 98 |
| LongSAGE tags | H. sapiens | 11,99 |
| Short RNAs | Caenorhabditis elegans | 100–102 |
| A. thaliana | 103 | |
| Testes-specific short RNAs | H. sapiens, M. musculus and Rattus norvegicus | 104–108 |
| MPSS | H. sapiens | 109 |
| Human Invitational, 41,118 full-length cDNAs | H. sapiens | 6 |
| FANTOM2, 60,770 full-length cDNAs | M. musculus | 110 |
| FANTOM3, 102,281 full-length cDNAs | M. musculus | 7 |
| Chromatin IP | ||
| ChIP–chip: p53, Sp1, cMyc | H. sapiens | 23 |
ChIP–chip:: NF B | H. sapiens | 24 |
| ChIP–chip:: RNA Pol II | H. sapiens | 111 |
| PET-sequencing-based: p53 | H. sapiens | 112 |
| CAGE, cap analysis of gene expression; ChIP–chip, chromatin immunoprecipitation and hybridization to DNA microarray; FANTOM, Functional Annotation of Mouse; MPSS, massively parallel signature sequencing; PET, paired-end ditag; polyA, polyadenylated; SAGE, serial analysis of gene expression. | ||
Download file
If the slide opens in your browser, select "File > Save As" to save it.

B