Abstract

Accurate and comprehensive sequence coverage for large genomes has been restricted to only a few species of specific interest. Lower sequence coverage (survey sequencing) of related species can yield a wealth of information about gene content and putative regulatory elements. But survey sequences lack long-range continuity and provide only a fragmented view of a genome. Here we show the usefulness of combining survey sequencing with dense radiation-hybrid (RH) maps for extracting maximum comparative genome information from model organisms. Based on results from the canine system, we propose that from now on all low-pass sequencing projects should be accompanied by a dense, gene-based RH map-construction effort to extract maximum information from the genome with a marginal extra cost.

Author affiliations

1. Christophe Hitte, Catherine Priat, Fabrice Senger, Thomas Derrien, Gwenaelle Evanno, Edouard Cadieu, Kristelle Lourgant, Sandrine Tacher, Stéphane Dréano, Nadia Berkova, Catherine André and Francis Galibert are at the CNRS, UMR 6061, Génétique et développement, Faculte de Médecine, 35043 Rennes Cedex, France.
2. Jennifer Madeoy, Travis D. Lorentzen, Christina Ramirez, Barbara Pullar, Vinay Oza and Elaine A. Ostrander are at the Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
3. Elaine A. Ostrander is also at the NHGRI/NIH, 50 South Drive, MSC 8000, Building 50, Room 5351, Bethesda, Maryland 20892-8000, USA.
4. Ewen F. Kirkness and Claire Fraser are at The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA.
5. Dan Thomas, Carol Scott and Panagiotis Deloukas are at the Wellcome Trust Sanger Institute, Hixton, Cambridgeshire, UK.
6. David B. Jaffe and Kerstin Lindblad-Toh are at the Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02141, USA.
7. These authors and their laboratories contributed equally to this work.

Correspondence to: Elaine A. Ostrander^2,3 Email: eostrand@mail.nih.gov

Correspondence to: Francis Galibert¹ Email: francis.galibert@univ-rennes1.fr

Published online 12 July 2005

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