Key Points
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In response to muscle damage, specialized adult myogenic progenitors known as satellite cells activate a myogenic transcriptional programme, which is analogous to that induced during embryonic and fetal muscle development.
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Either MyoD or Myf5 is required for the commitment of skeletal myogenic cells; in the absence of both transcription factors, progenitor cells assume non-muscle fates.
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Pax3 and Myf5 function upstream of MyoD in embryonic myogenesis. Pax3 is required for the proliferation, survival and specification of stem cells in the pre-somitic mesoderm.
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Shh directly activates Myf5 transcription through specific Gli1 binding sites in the epaxial somite enhancer.
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Activation of several signalling pathways — including the Wnt, Hedgehog, BMP and Notch cascades — determines the balance between the determination, proliferation, survival and differentiation of muscle progenitors in the somite.
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Pax7 is required for the development of adult muscle satellite cells that function in the postnatal growth and repair of skeletal muscle fibers.
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MyoD is required for adult muscle regeneration by promoting the myogenic differentiation of satellite-cell derived myoblasts.
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The activation or modulation of signalling pathways that are implicated in embryonic and fetal muscle formation might provide a useful therapeutic strategy for the generation of muscle cells from adult stem cells either in vivo or ex vivo.
Abstract
Skeletal muscle has an intrinsic capacity for regeneration following injury or exercise. The presence of adult stem cells in various tissues with myogenic potential provides new opportunities for cell-based therapies to treat muscle disease. Recent studies have shown a conserved transcriptional hierarchy that regulates the myogenic differentiation of both embryonic and adult stem cells. Importantly, the molecules and signalling pathways that induce myogenic determination in the embryo might be manipulated or mimicked to direct the differentiation of adult stem cells either in vivo or ex vivo.
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Acknowledgements
The authors thank B. B. Olwin for generously providing anti-syndecan-4 antibody. P.S. is supported by a Doctoral Research Award from the Canadian Institutes of Health Research. M.A.R. holds the Canada Research Chair in Molecular Genetics and is a Howard Hughes Medical Institute International Scholar. This work was supported by grants to M.A.R. from the Muscular Dystrophy Association, the National Institutes of Health, the Canadian Institutes of Health Research, the Howard Hughes Medical Institute and the Canada Research Chair Program.
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Glossary
- MYOGENIC
-
Cells that are committed to become muscle.
- SATELLITE CELLS
-
Quiescent cells that are located between the basal lamina and the plasmalemma of the muscle fibre, which are the main contributors to postnatal muscle growth.
- COMMITMENT
-
The restriction of cells to a particular developmental fate before differentiation.
- DETERMINATION
-
The process of irreversible specification, whereby a cell becomes able to differentiate autonomously, even if placed in another part of the embryo.
- SPECIFICATION
-
The process by which cells acquire a fate.
- PARAXIAL MESODERM
-
The embryonic tissue layer that forms on either side of the notochord and gives rise to the somites, which develop into the muscles, bones and cartilage.
- LATERAL-PLATE MESODERM
-
Part of the mesoderm that forms several different tissues and organs, and is the source of various regulatory signals, such as the bone morphogenic proteins.
- FATE
-
What a cell and its progeny will give rise to in a later stage of development; cells become progressively more restricted in their fate as development progresses.
- MYOTUBES
-
Multinucleated cells that are formed when proliferating myoblasts exit the cell cycle, differentiate and fuse.
- MYOFIBRES
-
Muscle fibres that are formed by the maturation of myotubes, which can be classed as slow, intermediate/fast or fast.
- TERMINAL DIFFERENTIATION
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The formation of a specialized cell type through a process that is not normally reversible.
- OSTEOGENIC
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Cells that are specified to become bone.
- ADIPOGENIC
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Cells that are specified to become adipose or fat tissue.
- CHONDROGENIC
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Cells that are specified to become cartilage.
- SOMITIC ANGIOBLASTS
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Endothelial progenitors that arise in the somite.
- MULTIPOTENTIAL STEM CELL
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A cell that has an intrinsic capacity to give rise to more than one differentiated cell lineage.
- PROGENITOR COMPARTMENT
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A population of cells that can give rise to more specialized cell types during development and differentiation.
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Parker, M., Seale, P. & Rudnicki, M. Looking back to the embryo: defining transcriptional networks in adult myogenesis. Nat Rev Genet 4, 497–507 (2003). https://doi.org/10.1038/nrg1109
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DOI: https://doi.org/10.1038/nrg1109
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