FIGURE 2 | The zebrafish vasculature.

From the following article:

Arteries and veins: making a difference with zebrafish

Nathan D. Lawson & Brant M. Weinstein

Nature Reviews Genetics 3, 674-682 (September 2002)

doi:10.1038/nrg888

Arteries and veins: making a difference with zebrafish

A | The development of the main trunk blood vessels in zebrafish. a, b and c | camera lucida drawings of a lateral view of zebrafish embryos at 12 hours post-fertilization (hpf), 18 hpf and 26–30 hpf, respectively; the position of the angioblasts or blood vessels is indicated in green in panel c. d | At 12 hpf, angioblasts arise in the lateral mesoderm and migrate towards the midline (as indicated by the arrows). e | By 18 hpf, cords of angioblasts can be seen that ultimately form the dorsal aorta and posterior cardinal vein. (d and e show dorsal posterior views of 12 and 18 hpf embryos, respectively, that have been hybridized with the vascular marker fli1.) f | By 24 to 30 hpf, the dorsal aorta and posterior cardinal vein have distinct lumens and carry circulating blood cells. A lateral view of a 24-hpf embryo after whole-mount in situ hybridization with a fli1 probe, showing the dorsal aorta (DA) and posterior cardinal vein (PCV). g | A cross-section through the trunk of a 30-hpf embryo, in which the lumens have already formed in the single dorsal aorta and posterior cardinal vein. Also shown are adjacent structures, such as the notochord and somites, the sources of Shh58 and Vegf32, 64, respectively, both of which are important in arterial differentiation (see text). B | The circulatory system of a zebrafish embryo at 30 hpf (lateral and dorsal views). Blood leaves the heart (H) and enters the lateral dorsal aortae (LDA), which converge into the dorsal aorta (DA). The circulation progresses into the caudal artery (CA), where blood empties into the caudal vein (CV) and returns rostrally through the common cardinal vein (CCV), which is also referred to as the Duct of Cuvier (DC), before returning to the heart. At this time, intersegmental vessels are sprouting from the dorsal aorta, but do not yet carry circulation. The grey box indicates the region of the embryo shown at 24–30 hpf in panel Af. fli1, friend leukaemia integration 1; ISV, intersegmental vessel; Shh, sonic hedgehog; Vegf, vascular endothelial growth factor.

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