One limitation of DNA sequencing platforms is that a proportion of sequencing capacity is often 'wasted' on uninformative sequence reads. Loose et al. developed 'Read Until' software for use with the Oxford Nanopore Technologies MinION DNA sequencer. In nanopore sequencing, a DNA strand translocates through a protein pore as electrical current is passed through, and the resulting shifts in the current (known as squiggles) are decoded into DNA sequence. The Read Until software analyses, in real time, the squiggle data generated from each pore in the machine, comparing them with simulated squiggle data of the known species of DNA being sequenced. Based on whether the first 250 squiggle events represent predetermined regions of interest, the pores can be individually controlled to continue sequencing or to have their current switched to reject that DNA molecule ready for a different one. For phage λ DNA, Read Until allowed selective sequencing of chosen regions, and it normalized sequencing coverage by rejecting regions that became over-represented during sequencing.