Nature Reviews Genetics 11, 31-46 (January 2010) | doi:10.1038/nrg2626

Article series: Applications of next-generation sequencing

Sequencing technologies — the next generation

Michael L. Metzker1,2  About the author


Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.

Author affiliations

  1. Human Genome Sequencing Center and Department of Molecular & Human Genetics, Baylor College of Medicine, One Baylor Plaza, N1409, Houston, Texas 77030, USA.
  2. LaserGen, Inc., 8052 El Rio Street, Houston, Texas 77054, USA.

Published online 8 December 2009


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