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Nature Reviews Genetics 10, 282–283 (1 May 2009) | doi:10.1038/nrg2584

Gene regulation: A new toolbox for mapping regulatory sites

Meera Swami

Large-scale experimental approaches for identifying sites of protein–DNA interaction are mainly based on chromatin immunoprecipitation (ChIP), which relies on prior knowledge of regulatory factors and can only interrogate sites for one protein at a time. Two new studies overcome these limitations by exploiting the simple principle that regulatory proteins protect their underlying DNA sequences from enzymatic digestion.