Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

Author affiliations

1. Zhong Wang and Michael Snyder are at the Department of Molecular, Cellular and Developmental Biology, and Mark Gerstein is at the Department of Molecular, Biophysics and Biochemistry, Yale University, 219 Prospect Street, New Haven, Connecticut 06520, USA.

Correspondence to: Michael Snyder¹ Email: michael.snyder@yale.edu

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