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The complement system includes more than 30 component proteins, regulators and receptors, present in plasma and on cells, which collaborate to provide defence against infection and to clear toxic materials. Complement is a key part of innate immunity and a modulator of the adaptive immune response; inherited deficiencies in complement proteins predispose individuals to bacterial infection and/or immune complex disease. The powerful cell-targeting and cell-killing properties of complement can be turned against self, a scenario that is prevented by the abundant expression of regulator proteins. In health, these regulator proteins maintain a fragile truce in which complement is continuously activated in what is known as tickover. Bacteria or other foreign surfaces lacking regulator proteins provoke massive activation and amplification of complement, generating activation products that label the invader, attract and activate phagocytic cells and directly cause lytic pathogen killing. Complement comprises a cluster of activation pathways that are triggered in various ways and progress through several amplifying enzymes to converge on a final common cell-killing pathway1,2. Surface-bound antibodies trigger the classical pathway, bacterial sugars initiate the lectin pathway and amplification is provided by the alternative pathway, regardless of initiating route (Fig. 1). Fluid-phase regulators prevent uncontrolled activation in plasma, whereas membrane-bound regulators restrict activation on cells. The protective (and pathological) effects of complement are mediated by its activation products — active fragments or complexes of complement proteins. C3, the most abundant complement protein, has numerous activities; some C3 fragments (for example, C3b, iC3b and C3dg) can covalently bind to targets and act as flags to instruct phagocytes to bind to and engulf the targets, or they can enhance humoral immunity by facilitating B cell activation. The small C3a fragment, among its many roles, can attract and activate phagocytes. Downstream of C3, the terminal pathway releases the proinflammatory fragment C5a and assembles a pore in target membranes — the membrane attack complex (MAC) — that effects the lysis of bacteria and other activating cells.

Figure 1: Targets for inhibition in the complement pathway.
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The figure shows a highly simplified view of the complement system and highlights the targets for pathway inhibition. Activation is triggered through classical (antibody) or lectin (sugar) pathways that rapidly converge to form a complement C3-cleaving enzyme (C3 convertase), C4b2a. The alternative pathway can be independently activated to generate its own C3 convertase (C3bBb) but, more importantly, amplifies activation regardless of trigger. C3 fragments, both soluble and surface-attached, engage specific receptors on expressing cells to mediate key activities. The C5 convertase, formed by the recruitment of an additional C3b into the C3 convertase, cleaves C5 to release a small fragment, C5a, which binds to receptors on expressing cells to mediate activation events. Formation of C5b initiates the membrane attack pathway; sequential recruitment of components C6, C7, C8 and C9 creates a pore in the target membrane – the membrane attack complex (MAC) – that can activate or kill the targeted cell. The complement system presents many targets for inhibition with drugs. In the activation pathways these include the initiating complexes and enzymes, the initiators of the alternative pathway loop and the C3 convertases. In the C3–C5 axis, potential targets include the individual components (for example, C3 and C5), the activation fragments (for example, C3a and C5a) and the C5 convertases. In the terminal pathway, agents might target individual components (such as C5, C6 or C7) or intermediates (such as C5b6 and C5b67), block the functional MAC pore or inhibit the downstream signalling events that mediate cell activation or destruction.

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The list of diseases in which complement has a role, either primary or secondary to other triggers, is long and growing (Table 1). Genetic deficiencies in complement components are relatively rare and associated with infections and immune complex diseases (Box 1); however, excessive or dysregulated complement activation contributes to many inflammatory, autoimmune and degenerative diseases, and is a much larger problem3,4. In a minority of these diseases, genetic changes, mutations or polymorphisms in complement proteins, regulators or receptors are the direct cause of dysregulation and disease; in most of these diseases, complement is not the primary cause but is recruited in response to tissue damage and serves to amplify and exacerbate inflammation and injury. Crucially, a small but growing group of conditions are now widely recognized as diseases caused by complement dysregulation, presenting obvious targets for complement-inhibiting drugs5,6. Lessons from disease-associated polymorphisms and highly penetrant disease-causing mutations in complement proteins have pinpointed the pathways and components driving pathology and guided the development of therapies for the numerous disease indications in which complement has an important role (Table 1).

Table 1 Complement and disease*

The merits of anti-complement therapy in preclinical models of disease have been explored in hundreds of papers; the earliest examples date back more than 50 years and describe the use of cobra venom factor (CVF) to inhibit disease in animal models7,8. Despite this long history, few drugs have entered clinical trials, and fewer still have progressed beyond Phase I. The recent interest in complement and disease has been fuelled by a number of factors — the demonstration of genetic associations that firmly link complement to common diseases was a key finding, but the biggest factor has been the success of a single drug, eculizumab (Soliris; Alexion Pharmaceuticals), in an orphan application: treatment of paroxysmal nocturnal haemoglobinuria (PNH) (Boxes 1,2).

PNH was among the first diseases to be treated with anti-complement therapeutics (Box 2); however, PNH is rare, and the identification of a much more common disease with complement dysregulation at its root was required to catapult complement into the spotlight. Age-related macular degeneration (AMD) is the commonest cause of blindness in the Western world; a perfect storm of genetic, pathological and clinical evidence has demonstrated beyond doubt that complement dysregulation is a crucial driver of inflammation and tissue damage in AMD and has catalysed an explosion of interest in controlling complement9,10. As a consequence, what began as a small industry targeting PNH and a few other orphan diseases has, over the past decade, grown into a major endeavour involving numerous companies ranging from small biotechnology companies to large pharmaceutical organizations.

As the field has grown, it has become increasingly clear that there will be no 'one size fits all' solution to complement therapies; agents that are effective in one disease might do nothing in, or even exacerbate, another. Rational design and selection of therapies will require in-depth understanding of the way complement works in each disease. In this Review, we consider the diverse disease targets, the many intervention points, the challenges associated with drugging these targets, and the multiple approaches to inhibition that are emerging as complement moves into the clinical mainstream. Below, before exploring pathology, we briefly revisit physiology.

Physiological roles of complement

Complement is a key part of innate immunity because it interacts with many other processes; for any therapy that seeks to block or modulate complement, the potential effects on these physiological roles must be considered to avoid iatrogenic injury.

Defence against infection. Patients deficient in complement proteins usually present with recurrent bacterial infections. C3 is a crucial source of opsonins, which label bacteria for removal by phagocytes; deficiency of C3 is rare and always marked by severe recurrent infections3. Patients deficient in classical pathway or lectin pathway proteins (C1, C4, mannose-binding lectin (MBL), MBL-associated serine protease 1 (MASP1) and MASP2) may also present with recurrent bacterial infections, and deficiencies of alternative pathway components or the positive regulator properdin also predispose to infection, although these deficiencies are associated with a strong bias towards Gram-negative bacterial infections. Deficiencies in terminal pathway proteins specifically predispose to infections with Neisseria species, typically those causing meningococcal meningitis or sepsis, as a consequence of the peculiar sensitivity of these pathogens to lysis by the MAC.

Protection against immune complexes. Immune complexes comprise antibodies bound to target antigens (cell debris) in a multi-molecular complex. Unhindered, immune complexes grow by acquisition and aggregation, eventually becoming large, insoluble aggregates that lodge in capillary beds and trigger inflammation and tissue damage. In healthy individuals, immune complexes activate the classical pathway and become coated with C1 and fragments of C4 and C3; these proteins mask antigens in the immune complex and disrupt the lattice, thereby limiting growth of the aggregates and simultaneously providing ligands for the receptors (complement receptor type 1 (CR1)) on erythrocytes, which sequester immune complexes, and for the receptors (CR1, CR3 and CR4) on phagocytic cells, which engulf and destroy immune complexes11. Patients with deficiencies in classical pathway components (in particular C1 and C4) usually present with an immune complex disease that closely resembles systemic lupus erythematosus (SLE), itself a disease of complement dysregulation12.

Priming adaptive immunity. The role of complement in stimulating the adaptive immune response was first demonstrated in complement-depleted mice 40 years ago13. Antigens coated with complement activation fragments provoke markedly greater antibody responses than do those that are uncoated; the C3dg fragment is crucial to this effect, ligating CR2 on B cells to deliver a powerful co-stimulatory signal that reduces the threshold for B cell receptor triggering and increases the amplitude of the response14. The smaller fragments of complement activation, C3a and C5a, enhance the ability of antigen presenting cells (APCs) to present antigens and stimulate T cell proliferation; together, complement products in the inflammatory microenvironment markedly influence T cell activation and the balance between effector and regulatory T cells15.

Other physiological roles. Many other roles of complement continue to emerge, including directing haematopoietic stem cells to marrow niches, regulating triglyceride uptake and storage in adipose tissue and the regeneration of damaged tissue after injury and during wound healing16. Each role in itself could warrant a review; therefore, here we merely highlight that these many roles may be affected by complement inhibitors.

Anti-complement drugs. Anti-complement drugs have the potential to affect each and all of the physiological roles discussed above (Fig. 1). It is inevitable that a drug that blocks any of the complement pathways will increase the risk of infections, either non-selectively or for certain groups of organisms. Any drug that stops activation of the classical pathway will affect the clearance of immune complexes and apoptotic cells. Inhibition of the activation pathways may disrupt an individual's capacity to mount an adaptive immune response, although therapeutic inhibition later in life is less detrimental as adaptive immunity is established and developed in older individuals. Anti-complement drugs may also disrupt normal lipid metabolism or interfere with the healing and resolution of injuries. All of these potentially damaging effects need to be considered when deciding whether and when to use an anti-complement drug and which part of the system to target. With appropriate prophylactic measures such as immunization and antibiotic therapy, and with careful consideration of the target, most of these effects can be managed.

Avoiding toxicity

A limiting factor in the use of anti-complement drugs has been an understandable concern regarding the harmful consequences of blocking the important physiological effects of complement described above, which are graphically illustrated in individuals with complement deficiencies. Deficiencies in the classical pathway prevent efficient clearance of immune complexes and apoptotic cells, leading to a high penetrance of lupus (93% penetrance in individuals with a C1q deficiency and 75% penetrance in individuals with a C4 deficiency)17, whereas individuals who are deficient in C3 or components of the alternative pathway or the terminal pathway have recurrent infections that can be life-threatening18. Although these risks can be managed with good clinical care, a guiding principle for any drug should be to cause as little disruption of physiological roles as possible, particularly if treatment is to be continued in the long term.

Researchers have devised several strategies to inhibit complement without risking iatrogenic injury: complement can be inhibited late in the pathway, wherein the only infection risk is from Neisseria species; complement can be inhibited transiently, which is sufficient for acute conditions, with minimal risk of immune complex disease; or therapy can be delivered directly to disease sites such that systemic inhibition is avoided. These strategies have spawned drugs that have entered the clinic, in some instances with dramatic effect. The C5-specific monoclonal antibody (mAb) eculizumab prevents MAC formation; opsonization is thus unaffected by eculizumab treatment and the risk of meningococcal infection can be mitigated through vaccination and antibiotics. The C3 blocker compstatin and an antibody antigen-binding fragment (Fab) directed against Factor D (FD), administered intravitreally, have undergone clinical trials for AMD19,20. The compstatin derivative APL-2 (Apellis Pharmaceuticals; a long half-life form) has just entered Phase I trials for add-on to standard-of-care in PNH; it will be interesting to see how systemic administration affects the risk of infection. Chimeric agents, comprising a complement ligand-targeting modality linked to a complement inhibitor, localize activity at the disease site. This concept has shown promise in murine models and has been developed as a therapy for PNH21,22. For any anti-complement drug, short-term inhibition for acute indications in a hospital setting is unlikely to incur serious risk of immune complex disease, and infection risk can be managed with tailored antibiotic cover.

Specific issues for complement-targeted drugs

Dosing. Experience gained from the first few drugs entering the clinic has added knowledge and identified issues that are specific to complement-targeted therapies. One major issue is dosing; most complement proteins are abundant in plasma and turn over rapidly, so adequate dosing of an inhibitor can be challenging. For example, C3 is present in plasma at ~1 g per l, and ~10–20% of total plasma C3 (~300–1000 mg) is consumed and replaced daily simply through the normal tickover process23. It is therefore obvious that large doses of complement-targeting drugs and frequent administration will be needed to block complement at the level of C3. Dosing is an issue for current drugs; the Cinryze (a plasma-derived C1 inhibitor; Shire Pharmaceuticals) dose for prophylaxis in hereditary angioedema (HAE) is 1,000 units (100–250 mg) delivered intravenously every 3 days; the eculizumab maintenance dose for adults with PNH is 900 mg every 2 weeks24,25, whereas in atypical haemolytic uremic syndrome (aHUS) the maintenance dose is 1,200 mg every 2 weeks. These huge and frequent doses contrast starkly with agents targeting cytokines, which are released de novo in disease and at much lower levels; for example, the tumour necrosis factor (TNF)-specific mAb adalimumab (Humira; AbbVie) is effective in rheumatoid arthritis at a dose of 40 mg every 2 weeks26. The plasma C5 concentration is ~80 mg per l and the turnover rate is ~60 hours27; as a consequence, even with such high doses, breakthrough activity can occur in some patients treated with eculizumab and monitoring of complement activation in plasma is required28. Alexion have recently launched two new agents that are in Phase I trials (ALXN1210 and ALXN5500; 'next-generation' eculizumab molecules) that were probably developed to overcome some of the issues with eculizumab. C5 is not limiting in the complement cascade; therefore, inhibition at the C5 stage requires near-complete blockade or depletion of C5; these threshold kinetics necessitate large doses to achieve an effect. Approaches to circumvent the problem of high levels of protein turnover have been described, using drugs that only bind to activated forms of complement proteins, which are present at much lower concentrations in plasma29,30; these approaches show potential but have not been tested in humans.

Apart from the problems arising from the sheer abundance of complement proteins, dosing is complicated because plasma complement levels vary widely in the population and because many are acute phase reactants, with synthesis increasing markedly in inflammation, which sometimes causes plasma levels to rise even in the face of increased consumption31. The plasma concentration of FD is relatively low (~5 mg per l) but it is turned over extremely rapidly (~1.33 mg per kg per day)32; it can therefore be predicted that large amounts of a drug will be needed to effectively inhibit FD in vivo. Studies in non-human primates support this — a 5 mg per kg intravenous dose of a FD-specific Fab (lampalizumab, formerly called FCFD4514S, Roche/Genentech) inhibited the alternative complement pathway for only 3 hours; in that time the plasma levels of FD increased 10-fold, which is probably reflective of the retention of FD–lampalizumab complexes in the plasma33. Lampalizumab, administered intravitreally, is currently in Phase III trials in AMD.

Owing to their chemical nature, small-molecule anti-complement agents tend to have short half-lives in vivo. This is not necessarily a limitation, as full coverage can often be achieved through repeated dosing in situations of long-term therapy. Daily subcutaneous dosing is manageable and is likely to be acceptable for patients with life-threatening diseases; oral bioavailability can often be achieved with these agents, which is a key advantage. In some conditions — such as traumatic brain injury, myocardial infarction, transplantation, stroke and other ischaemia–reperfusion injuries — transient complement inhibition for a few hours may be sufficient to prevent tissue damage. In these cases, small molecules have an added benefit in that once dosing is ceased, rapid clearance leads to speedy recovery of complement activity, restoring its infection-fighting and tissue-repairing properties. When developing small-molecule drugs for specific targets, their mode of action is crucial and target tractability becomes a key consideration; in some instances, the small size of the drugs may become a limitation, for example in blocking protein–protein interactions (see below). When a drug with a longer half-life is needed, agents such as antibodies come to the fore. Alternatively, small molecules may be modified to increase their mass by pegylation or by coupling to domains that extend their half-life in vivo: for example, albumin-binding or antibody crystallisable fragment (Fc) domains34,35.

In some diseases, complete inhibition of complement may not be needed for efficacy; agents such as soluble CR1 (sCR1; also known as TP10, Celldex Therapeutics) that downmodulate the complement enzymes through dynamic activities (such as accelerating their decay or increasing cofactor availability) can mediate dose-dependent partial inhibition of complement rather than the sharp threshold effect typical of blocking antibodies36,37. Such agents may enable therapy at scalable doses and in a safe and effective manner; however, the fact that even large biological agents such as sCR1 have relatively short half-lives will affect the choice of indication.

Getting to where the action is. Many pharmaceutical companies are now focused on the development and manufacture of biologics because of their historical success, good economic return and low rate of attrition. As a consequence, most anti-complement agents developed to date are biologics — either antibodies or recombinant proteins — that deliver inhibitory activity. Essential to all drugs is their ability to get to the sites of disease; some complement-mediated diseases manifest in the vasculature (for example, PNH and vasculitis), whereas in other diseases (such as AMD, neuromyelitis optica and arthritis) complement attacks tissues at sites remote from systemic circulation. The ability of systemically-administered drugs to diffuse to the site of tissue damage and inflammation will thus be essential for treatment success. The liver produces most of the complement components found in the circulation, and if diffusion of circulating components to disease sites drives inflammation and disease then systemic depletion or blocking of specific complement components may ameliorate the pathology. However, locally produced complement is known to have key roles in some indications. For example, in renal allograft rejection, locally produced and activated C3 drives the T cell response and immune rejection38; in this case, systemic anti-C3 therapy will be ineffective unless the drug gets into the tissues. Similar challenges exist in organs protected by tight barriers, such as the nervous system and the eye. In some diseases these barriers become damaged and leaky with disease progression, but it is likely that complement activation contributes to the early, pre-inflammatory stages of eye and brain diseases, so to be of value complement-inhibiting drugs are required in the relevant organ early in the disease when the barriers are still intact. Assuming that they have good bioavailability, small-molecule drugs may have advantages over biologics in these contexts because of their higher tissue penetrance; however, of the numerous small-molecule anti-complement agents that have been developed and tested in humans, none has yet progressed to the market39–41.

Getting in the way. Complement is a good target for biologics because the cascade involves multiple protein–protein interactions and conformational changes, exposing large surfaces that lend themselves to large blocking drugs (Box 3). By contrast, for a small molecule to block a protein–protein interaction it must target crucial sites and specific surface features — for example, charged or hydrophobic pockets42. The wealth of structural information now available in the field, including snapshots of convertase enzymes and MAC precursor complexes captured in active conformations, unmasks the precise nature of these protein–protein interactions and identifies sites that are key to the interaction that can be targeted with small molecules or biologicals using structure-based drug design43,44. Alternatively, the expansive small-molecule libraries that are now ubiquitous in pharmaceutical companies have been successfully screened to reveal agents that bind to and inhibit interactions within the cascade through competitive or allosteric mechanisms45,46. Small peptides selected from such libraries for binding components or inhibiting complexes are now numerous in the literature; for example, the cyclic peptide compstatin, which blocks C3 from binding to the convertase, has already entered clinical trials45,47.

Target validation in models. Validation in rodent models of disease can be challenging or even misleading, and care needs to be taken to ensure that the disease mechanism in the chosen model is the same as that in the human disease. To illustrate the differences in complement systems between mice and humans, consider the receptors for C3 fragments. In humans, CR1 and CR2 are encoded by separate genes and have different roles: CR2 binds to C3dg and enhances humoral immunity whereas CR1 acts as a cofactor for the FI-mediated cleavage of C3b and has a key role in transporting immune complexes48. In mice, CR1 and CR2 are generated from the splicing of one gene and have limited expression; the main cofactor for C3b cleavage and generation of C3dg in the mouse is CR1-related gene/protein Y (CRRY), a broadly expressed protein absent in humans49. Therefore, mice and humans respond to C3 fragments through different receptors and with different outcomes that will probably alter the effects of C3-targeted drugs. Nevertheless, in many models, disease mechanism and response to complement inhibition translate well and proof-of-principle is readily obtained. With current efforts to reduce animal use in scientific research, initiatives are emerging to better validate drug targets at an early stage of development without the use of animals, for example, using technologies such as microfluidic organs-on-chips50.

What does the current toolbox contain?

Before describing specific tools, we first provide some guiding principles and suggestions for the classification of anti-complement agents. There are several discrete ways in which an agent might act to influence complement (Fig. 1). An agent might inhibit progression in the activation or terminal pathways, act as an antagonist of an activation product, or cause activation and consumption of complement. Another broad distinction can be drawn between those drugs that inhibit complement systemically and those that are directed to specific sites of pathology to localize their inhibitory activity. Anti-complement drugs can also be divided into categories based upon their molecular characteristics: purified or recombinant forms of naturally occurring human regulators; modified protein copies or mimics of natural regulators; copies of pathogen-derived regulators; mAbs or fragments of mAbs; naturally occurring or synthetic small-molecule inhibitors; or antisense or similar nucleic acid constructs. Finally, anti-complement agents can be grouped according to the part of the system they target, a convention that we follow in this Review.

Targeting the binding and assembly of the initiating complex. Initiation of classical or lectin pathway activation involves the recognition of an activating surface; in the classical pathway this activation occurs through a surface-bound antibody that ligates C1q, whereas in the lectin pathway this occurs via specific sugars on the surface that bind to MBL or collectins (Fig. 1). Agents that block these events would switch off the respective activation pathways at the very first step, an attractive prospect given the amplifying nature of the complement system51. A potential classical pathway inhibitor might block C1q-binding sites on the antibody or the antibody-binding sites on C1q. A lectin pathway inhibitor might mask surface MBL-binding sugars or the sugar-binding sites on MBL. In either case, the agent would need to bind the target with higher affinity than the physiological ligand to compete effectively. Alternatively, an agent could disrupt or prevent the assembly of the multi-molecular complex, which comprises the recognition unit (C1q and MBL in the classical and lectin pathways, respectively) and the associated enzymes (C1r and C1s in the classical pathway and MASP1 and MASP2 in the lectin pathway) that are essential for pathway activation52. Both complexes circulate preassembled in plasma, so any agent targeting the complex would need to dislodge the bound enzyme to be effective; surprisingly, the C1s- and MASP2-targeting mAbs from True North and Omeros, respectively (described below), both achieve this effect. Another approach to inhibiting activation of the complexes would be to develop a molecular lock that restricts the conformational changes in C1q or MBL that occur upon ligand binding and are essential for activation of the associated enzymes. Such approaches have been used successfully to develop small-molecule inhibitors of chemokine receptor activation53,54, but have not yet been reported for C1 or the MBL–MASP complex.

Several C1q function-blocking mAbs have been reported and were recently shown to be effective in a murine model of the antibody-mediated demyelinating disease, neuromyelitis optica55. Numerous peptide blockers of antibody–C1q interactions have been described, derived from the sequences of C1q binding sites in the antibody, C1q receptors or C1q itself, or selected from libraries. One such peptide blocker, termed peptide 2J, is a powerful inhibitor of the classical activation pathway in multiple species56; however, there have been no further reports of this agent. Other peptides have been described that interfere with the binding of C1r and C1s in the C1 complex, but none has progressed beyond in vitro studies. The binding of MBL to specific sugar residues on surfaces presents a potential drug target, but no agents acting in this manner are reported. A recent publication describes a virus-derived peptide, peptide inhibitor of complement C1 (PIC1), that binds to the collagenous regions in both C1q and MBL and inhibits both the lectin and classical pathways in vitro and in vivo in rodents57.

Targeting the enzymes of the initiating complexes. The enzymatic activity of the classical or lectin pathway-initiating complexes is provided by the serine proteases C1s and MASP2, respectively. In the C1 complex, immunoglobulin binding induces conformational changes in C1q, triggering auto-activation of C1r that in turn cleaves and activates C1s in the same complex. The precise steps involved in activation of the MBL–MASP complex are less clear but probably involve a similar multi-step process58. The serine protease enzymes in these complexes are potential targets for pharmacological inhibition. Both the pharmaceutical industry and nature itself have invested heavily in the development of serine protease inhibitors (SPIs), and numerous SPI drugs have been made, of differing specificities and compositions, to target the myriad serine proteases essential for digestion, haemostasis, immunity, reproduction and many other physiological and pathological processes. The biggest problem in designing an SPI drug to inhibit a particular protease is specificity — almost all SPI drugs have some 'off-target' effects that can limit their use. Even nature struggles — the sole natural inhibitor of C1r and C1s is an SPI, C1 inhibitor (C1INH; also known as SERPING1), but C1INH also controls MASPs and proteases in the coagulation and kinin systems. Indeed, deficiency of C1INH leads to dysregulation in all these proteolytic cascades that, in aggregate, cause the disease HAE59,60. C1INH removes activated C1r and C1s from C1q to form a stable complex in which C1INH itself is cleaved and inactivated.

C1INH has a long history of use as a drug; indeed, it can claim to be the 'first-in-man' complement drug and the first of the natural inhibitors to be recognized as a potential therapeutic. More than 40 years ago, patients with HAE were shown to be deficient in C1INH and to respond to plasma replacement, provoking efforts to purify C1INH for treatment of acute attacks59,61. Remarkable successes in these early studies rapidly led to the adoption of plasma-derived C1INH as the standard of care for acute episodes in HAE. The obvious advantage of this approach is that a plasma-derived molecule should be low risk (once infectious agents are eliminated) and non-immunogenic. The widespread availability of industrial-scale protein-fractionation facilities — established for the production of immunoglobulins, albumin and other plasma products — made this a viable approach for purifying C1INH. Unfortunately, concerns about viral transmission in plasma-derived proteins have led to its removal from the clinic in several countries, including the United States. Recent methodology improvements and new approaches to production of C1INH have changed this unsatisfactory situation; two ultra-pure plasma-derived C1INH products, Cinryze and Berinert (CSL Behring) are now approved by the US Food and Drug Administration (FDA) and other regulatory agencies. Some companies invested in better methods of purifying plasma C1INH, whereas others set about making it. A full-length recombinant C1INH was first reported a decade ago. This agent, termed Ruconest (Salix Pharmaceuticals), has been approved for therapy of acute attacks in Europe and, very recently (July 2014), also in the United States.

Early administration of C1INH reduces the duration and severity of acute attacks, findings confirmed in the large IMPACT 2 study of over 1,000 attacks62. Its use in prophylaxis is more controversial; a subset of HAE patients have frequent and severe attacks despite therapy with danazol (an anabolic steroid that increases C1INH synthesis) and/or ε-aminocaproic acid (a protease inhibitor) or cannot tolerate these agents. Regular intravenous administration of C1INH (every 3–7 days) to restore plasma levels is an effective therapy in this group63,64; however, in the United States it is now becoming accepted practice to treat all HAE patients in this manner, a practice limited elsewhere by cost and lack of evidence base65. Berinert has been approved for on-demand, patient-administered therapy in the United States and Europe.

Given its long history and known safety and efficacy in HAE, it is surprising that C1INH has not been more widely used in other conditions characterized by complement dysregulation. There are a number of published studies, mostly small, demonstrating that administration of C1INH reduces mortality in sepsis, and in some studies the effect is startling66,67. Other studies have reported positive effects of C1INH in myocardial infarction and in ischaemia–reperfusion injuries in both animal models and humans, but no large-scale clinical trials in these other applications have been published68,69.

Several broad-spectrum protease inhibitors already in clinical use have C1r, C1s and MASPs among their targets; perhaps the best example of these is nafamostat mesilate (also known as Futhan or FUT-175), a small-molecule protease inhibitor used to treat disseminated intravascular coagulation and acute pancreatitis that affects multiple plasma protease systems39. However, the many off-target effects and resultant toxicity of nafamostat mesilate make it a poor candidate for regulating complement in vivo. Other small-molecule inhibitors of C1s of varying degrees of specificity and strength have been described, including C1s-INH-248 (BASF Pharma) and BCX-1470 (BioCryst Pharmaceuticals), both no longer in development, and recently a family of biphenylsulphonyl thiophene derivatives that, when pegylated, displayed strong C1s inhibition and promising pharmacokinetics70. Modern methods of structure-based design offer the prospect of much more selective small-molecule inhibitors of the initiating complex enzymes, as demonstrated for other proteases71. Indeed, the 'directed evolution' of a sunflower-derived peptide, trypsin inhibitor 1, created specific inhibitors of MASP1 and MASP2 that await testing in vivo72.

An inhibitory mAb against C1r and C1s or MASPs might address the specificity issue that limits the clinical use of small-molecule agents. A mAb against C1s, TNT003 (True North Therapeutics), prevented erythrocyte haemolysis in an ex vivo study of autoimmune haemolytic anaemia41; a humanized analogue of this mAb, TNT009 (True North Therapeutics), is being fast-tracked to clinical trials in this disease. A recombinant mAb Fab was recently described that bound C1s with nanomolar affinity and potently inhibited classical pathway activation, but so far this has only been tested in vitro73. A MASP2-blocking human mAb (OMS721), which is an effective and long-lived (up to a week) lectin-pathway inhibitor that is delivered intravenously or subcutaneously, was recently awarded US FDA Orphan Drug status for use in aHUS and other thrombotic angiopathies and is currently in Phase II/III trials (NCT02222545). A recent (August 2015) Omeros press release described positive outcomes in aHUS from this trial.

Targeting the C3 convertases. The C3-cleaving enzymes of the classical, lectin and alternative pathways, C4b2a and C3bBb, are assembled in quite different ways despite their functional and structural similarities (Fig. 2). In the classical and lectin pathways, C4 is cleaved by activated C1s (or MASP2) in the surface-bound C1 (or MBL–MASP) complex; the large C4b fragment binds to adjacent surfaces via a nucleophilic attack on a thioester group, captures C2 and presents it for cleavage by C1s or MASP2 as above. The C4b2a complex is the C3 convertase of the classical and lectin pathways. C2a is a serine protease that cleaves C3 to form C3a and the opsonin C3b; C2a can also cleave C5 (when properly presented) to form C5a and C5b. These two complement proteins are the sole substrates of the C2a serine protease. Assembly of the alternative pathway C3 convertase requires C3b, generated through classical and lectin pathway activation or from tickover activation on surfaces. C3b binds to FB, triggering major conformational changes that expose FB to cleavage by FD, a unique plasma serine protease that has FB as its sole substrate. The C3bBb complex is the C3 convertase used in the alternative pathway; the Bb fragment, generated from FB cleavage, is a serine protease that cleaves C3 and C5 in precisely the same manner as C2a in the classical pathway. The key amplifying role of the alternative pathway makes it a very attractive source of drug targets that can be targeted in a variety of ways (Fig. 2).

Figure 2: Controlling the alternative-pathway amplification loop.
figure 2

The amplification (C3b feedback) loop is a positive-feedback cycle that consumes complement C3 to generate more enzyme and activation products; if unregulated, it cycles until all available C3 is consumed. Tight regulation is provided in the plasma by enzymes and cofactors that remove the C3 fragment C3b from the feedback cycle for breakdown into smaller fragments. As a consequence, the C3b feedback cycle normally operates at a very low rate (tickover). The balance between activation and regulation is disturbed in disease; a healthy balance can be restored by providing extra control (for example, increasing regulation, such as that provided by the complement regulatory protein Factor H (FH), thereby increasing 'feed out' from the amplification loop) or by preventing the formation of C3b in the feedback cycle (for example, by blocking convertase enzyme, thereby decreasing 'feed in' to the amplification loop). Agents that target amplification of complement can have major therapeutic effects. Ba, non-catalytic fragment of FB; C3b–FH, complex between C3b and FH; CR1, complement receptor type 1; iC3b–FH, complex between inactive C3b and FH. Adapted with permission from Ref. 157, Elsevier.

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It is obvious from the above description that the C3 convertases present a dizzying array of opportunities for regulation. Agents might target the assembly of one or more of the complexes, preventing complex formation and/or causing complexes to break up. They might target one or more of the serine proteases responsible for formation of the convertase (C1r, C1s, MASP2 or FD), convertase activity (C2a or Bb) or convertase regulation (FI). Alternatively, they might target the substrates of these enzymes, C3 or C5, by blocking binding to or cleavage by the convertase or acting as molecular locks to prevent conformational changes essential for activation. Agents might also target active fragments (C3a, C5a, C3b or C5b) to block their downstream effects. Indeed, nature has adopted some of these strategies to keep complement in check. The convertases are naturally labile and break up in a matter of minutes; regulatory proteins that accelerate this decay exist both in plasma and on membranes, and they function by binding to the complex and displacing the enzyme. Some of these regulatory proteins remain bound after decay and act as cofactors for the further cleavage and irreversible inactivation of C3b or C4b by the plasma serine protease FI.

Using the endogenous C3 convertase regulators as drugs or leads. The plasma regulators of the C3 convertases were obvious targets for drug development given the success of C1INH. To date, none of the other plasma regulators has entered the clinic despite a large amount of research and preclinical activity. In vitro studies more than 35 years ago showed that modest (around 10% above baseline) increases in the concentrations of the complement regulators FH and FI markedly reduced plasma alternative-pathway activity, provoking the suggestion that augmentation of these proteins might be of therapeutic benefit74; however, large (and probably frequent) doses will be required to substantially alter the levels of these abundant proteins. Despite this, there has been considerable recent interest in using plasma-derived FH as a therapy, particularly in those diseases strongly linked to FH polymorphisms and mutations. Deficiency of FH is rare and always associated with severe C3 dysregulation in plasma or on cell surfaces that leads to a range of renal diseases, including C3 glomerulopathy and aHUS; FH deficiency has been successfully treated with plasma exchange, strongly suggesting that FH would be an effective therapy in these diseases75. Indeed, in FH-deficient mice, therapy with human FH reversed renal injury76. AMD is strongly linked with the FH Y402H polymorphism, and this has provoked intense interest in giving at-risk individuals the protective Y402 form of FH to prevent or treat AMD. Several companies established programmes to produce plasma-derived or recombinant FH with this as the primary aim, but none of these programmes survive. Of note, some evidence from mouse models and human studies suggest that FH would need to be administered intra-ocularly in AMD to control complement dysregulation locally and alter the course of disease77,78.

In one study, instead of expressing full-length FH for use in therapy, this large molecule was stripped down to its key functional units and re-assembled to create a minimized FH, mini FH, which comprised four amino-terminal, complement-regulatory short consensus repeats and two carboxy-terminal, surface-binding short consensus repeats joined by a short linker. Mini FH proved to be an effective complement inhibitor in the fluid phase and on surfaces in multiple in vitro assays79,80, and it is now in preclinical development for therapy of PNH (AMY-201; see the Amyndas website). Other approaches aimed at targeting FH activity to surfaces are described in later sections.

C4BP, the fluid-phase regulator of the classical pathway C3 convertase, is a complex, oligomeric molecule that to date has received little attention in terms of therapeutic applications. C4BP deficiencies are extremely rare, restricted to a single case report81. In mouse models of arthritis, administration of plasma-derived C4BP reduced severity of disease82, but no human studies have been described.

FI is the key enzyme for regulation of the C3 convertases and deficiency causes complement consumption and consequent susceptibility to bacterial infections. Individuals with an FI deficiency present with repeated infections that may be life-threatening, and even heterozygotes may have an increased infection risk83. Plasma therapy to replace FI would, by analogy with FH deficiency, be expected to help, although a single small study found no clinical benefit84. A recent report showed that FI supplementation reduced complement activity in sera from individuals with more active complotypes, provoking the suggestion that FI therapy would reduce the risk of developing diseases linked to complement dysregulation85.

Properdin is a plasma protein that binds to and stabilizes the alternative pathway convertase; in other words, it acts as a positive regulator86. Properdin may also act as a pattern-recognition molecule, binding targets and initiating activation of the alternative pathway. Deficiency of properdin (X-linked) predisposes individuals to infections by bacteria, particularly Neisseria species. Supplementation of properdin in individuals who have a deficiency would be expected to reduce infection risk. A recent study suggested a broader role for properdin therapy — low-dose recombinant properdin markedly reduced susceptibility to bacterial infection in a mouse model, raising the possibility that supplementation might be effective in sepsis and other severe infections in humans87. The stabilizing role of properdin on the alternative pathway C3 convertase means that blocking this positive regulator may be therapeutic in inflammatory disease. A mAb targeting properdin (NM9401) and a small-molecule properdin antagonist have been reported by Novelmed Therapeutics for use in PNH and rheumatoid arthritis, respectively (see the Novelmed Therapeutics website).

Using the membrane C3 convertase regulators as drugs or leads. The membrane regulators of the C3 convertases present a different challenge compared to the plasma regulators in that they cannot readily be purified for testing as potential drugs. The only way to test whether a membrane regulator might work as a therapeutic is to make a recombinant soluble form. The first of these, described in 1990, was a soluble recombinant form of CR1 (known as sCR1 or TP10) comprising the entire extracellular sequence of thirty short consensus repeats (>1,800 amino acids). The protein was a powerful inhibitor of both classical and alternative pathway convertases in vitro in human and rodent sera; pre-treatment with sCR1 protected against reperfusion injury in a rat model of myocardial infarction88. Over the ensuing two decades, this agent provided an excellent proof-of-principle for complement inhibition in diverse models of human disease, effective in a wide range of disease models and in rodents, pigs and primates. After initial development work by SmithKline Beecham, Avant Immunotherapeutics took sCR1 into clinical trials in adult respiratory distress syndrome, acute lung injury and cardiopulmonary bypass; although some positive results were described37, there were also many failures, notably in large sepsis trials, and sCR1 has not progressed for these indications. More recently, guided by success in animal models, sCR1 entered clinical trials in dense deposit disease (CDX-1135; Celldex Therapeutics); regrettably, this study terminated in 2014 because of recruitment issues.

Numerous modified forms of sCR1 have been created to improve its therapeutic potential and/or simplify its production. TP20 incorporated multiple oligosaccharide sialyl Lewis X epitopes to bind to selectins, which are upregulated on endothelia at inflammatory sites. This simple change improved efficacy compared to unmodified sCR1 in rat ischaemia–reperfusion models89. The minimum complement regulatory unit from CR1 was known to be the three N-terminal short consensus repeats. A drug that comprised these three short consensus repeats attached to a nonspecific membrane-binding motif (APT-070; also known as Mirococept; AdProTech) proved remarkably effective as a complement inhibitor when delivered locally or systemically in rodent and pig models90,91. The agent was inexpensive to produce and proved safe in human studies. The MRC-funded EMPIRIKAL trial (UK Clinical Research Network (UKCRN) identifier: 16181), which is due to commence in 2015, tests the effect of Mirococept pretreatment of donor kidneys on their survival and function post-transplant.

Although recombinant soluble forms of the other membrane C3 convertase regulators, CD46 and CD55, have been produced and tested in animal models, most have stalled at the early preclinical stage. The exception was a chimeric CD55–CD46 molecule (CAB-2; also known as MLN-2222; Millenium Pharmaceuticals) that entered Phase I trials in cardiopulmonary bypass but was not progressed.

Inhibitors of the proteases involved in convertase formation and activity. There are eight protease targets that could be used for convertase regulation. The classical and lectin pathway initiating-complex enzymes (C1r, C1s, MASP1 and MASP2) are discussed above. Generation of the alternative pathway convertase requires FD, and deficiency of FD results in the absence of alternative pathway activity and marked susceptibility to bacterial infections92. The alternative pathway has a crucial role as an amplifier regardless of the initiating trigger, making it an attractive target for therapeutic modulation and bringing FD centre stage. The first FD-targeted therapeutic to reach the clinic, lampalizumab, is a humanized immunoglobulin G1 (IgG1) Fab fragment that inhibits FD activity by binding to the exosite to prevent substrate binding93. A published Phase I study of intraocular administration in patients with a severe form of AMD termed geographic atrophy (GA) demonstrated safety20. Phase II results of monthly treatments, announced but unpublished (MAHALO study), describe a 20% reduction in GA area compared to controls at 18 months; these startling results were even better in patients selected based on an undisclosed FI genetic biomarker, who showed a 44% reduction at 18 months. Phase III trials of therapy (10 mg intravitreal every 4–6 weeks) in patients with GA are ongoing.

Several broad-spectrum small-molecule protease inhibitors, including dichloroisocoumarin, bind to and inhibit FD; structures obtained from these complexes have identified unique properties of FD that offer the prospect of designing specific inhibitors94,95. Novartis recently reported the evolution of small-molecule inhibitors of FD that are active in the nanomolar range96. Very recently, Achillion have announced the development of orally active, potent and specific small-molecule inhibitors of FD that they propose for therapy in PNH. It remains to be seen whether the high natural turnover of FD will limit the use of these small molecules in humans.

A number of mAbs or mAb fragments have been described that inhibit FB cleavage in a variety of ways. One of these, termed mAb 1379, has been developed as a therapeutic by Taligen97; TA106 is a Fab fragment of mAb 1379 that blocks formation of the alternative pathway convertase. Taligen was acquired by Alexion in 2011 for US$111M, and the agent is currently undergoing humanization for further use in humans, with AMD and asthma as primary targets. Another FB-specific antibody, bikaciomab (NM9308; Novelmed), is also in development for use in AMD, and small molecule inhibitors of FB have been reported (in Novartis patents) although there is no evidence yet of their use in preclinical models of disease. Isis Pharmaceuticals have described the use of FB antisense oligonucleotides for therapy of murine lupus nephritis, but progression of this technique to humans has not been reported98. The functional homologue of FB in the classical pathway is C2; despite a more limited role in pathology, C2a-specific mAbs that block the classical pathway C3 convertase have been developed and patented by Tanox (now part of Genentech; Patent ref. WO 2001070818 A8); no trials are reported on these interesting drugs. An intriguing agent, a linear peptide (rH17d) derived from a Schistosoma species protein, bound C2 and inhibited C3 convertase formation, but has not been further developed to date99.

Targeting individual convertase components or regulators. Individual convertase components and regulators can be targeted in various ways to influence complement activity: components may be depleted in the plasma by increasing their consumption or decreasing their synthesis; regulator concentrations may be increased by supplementation or an increase in synthesis; components and/or regulators may be prevented from undergoing essential conformational changes or enzymatic cleavage; or components may be rendered more susceptible to spontaneous or accelerated decay.

One example of altering consumption is illustrated by CVF, a C3b analogue present in cobra venom that, when injected into mammals, binds to FB to form a stable C3 convertase that is resistant to normal regulatory processes and thus activates the system to exhaustion. This activity of cobra venom was recognized over a century ago, and purified CVF was first used as a tool to render the complement system of rodents deficient almost 50 years ago, making it the first anti-complement 'therapeutic' (Refs 100,101). Although the empiric use of cobra venom in therapy has a long history in everything from cancer to cosmetics, CVF as a drug has severe limitations: first because it is immunogenic, which renders repeat injections ineffective, and second because rapid and complete activation of complement by CVF generates high concentrations of C5a, causing circulating neutrophils to activate and aggregate in the lungs and other organs102. It is also unclear whether CVF can deplete complement locally in tissues. Attempts to address immunogenicity through humanization of CVF have created a hybrid that is 94% identical to human C3; small stretches of CVF-derived sequences in the C3 α-chain are sufficient to retain CVF-like complement consumption103. Although immunogenicity may be low, concerns over the toxic effects of continuous consumption of complement by CVF ensure that use will be restricted to acute conditions.

Supplementation of the plasma convertase regulators offers therapeutic possibilities above and beyond the restoration of plasma levels in rare cases of deficiency. Systemic modulation of the cascade using endogenous soluble regulators (or soluble versions of membrane-associated control proteins) is likely to cause downmodulation rather than complete blockade and effect therapy in a manner that is more measured. Efforts to modulate complement by administering extra FH or FI are described above. However, the amounts of protein needed to raise plasma levels sufficiently to affect convertase activity may be prohibitive; the feasibility of this approach will require careful dose prediction, and dosing will probably be disease-specific and perhaps individual-specific. Gene-therapy approaches to increase local synthesis are also now attracting attention — for example, the introduction of a construct that increases the production of FH or another complement regulator in the retina might prove effective in AMD, a disease in which other gene-therapy approaches have already been effective in models and have reached Phase I/II clinical trials104.

Decreased synthesis is the aim of a variety of antisense and related agents targeting complement components either systemically or locally, and these agents are the subject of a number of published patents. However, to date these strategies have only been tested in vitro and (for a minority) in animal models; for example, C6-targeted antisense oligonucleotides markedly reduced hepatic synthesis and plasma C6 levels, and ameliorated disease in a mouse model of traumatic brain injury105. C3 antisense is described in a number of patents over the past 10 years but has not progressed in vivo. Alnylam described an RNA interference (RNAi) molecule targeting C5, ALN-CC5, that reduced plasma C5 levels by >90% when administered monthly by subcutaneous injection to non-human primates; this agent, developed for therapy of PNH, is now in Phase I/II trials (as announced in a recent Alnylam press release).

Targeting the anaphylactic peptides. The polypeptides C3a and C5a, released owing to the action of the C3 and C5 convertase enzymes, are biologically active, and they bind specific receptors on phagocytes and many other cell types to exert effects. The range of cell targets and activities is large and reviewed well elsewhere106, but their pro-inflammatory activities are particularly relevant to disease. C5a is a powerful inflammatory trigger implicated in many diseases, whereas C3a exhibits a complex mix of pro- and anti-inflammatory activities107. Both C3a and C5a (and their metabolites) bind to seven-transmembrane-domain G-protein-coupled receptors (GPCRs), which are members of the large cytokine and chemokine receptor family. Similar to other GPCRs, those that bind C5a and C3a are attractive drug targets; many specific small-molecule blockers have been developed for cytokine receptors, although few have been developed for chemokine receptors, which more closely resemble the C3a anaphylatoxin chemotactic receptor (C3aR) and C5aR1 (Ref. 108). Dompé Pharmaceutical have described small-molecule C5aR1 blockers and claim potential for therapy of a wide panel of diseases; however, no trials are yet reported. Perhaps the most studied C5aR1 blocker is the cyclic hexapeptide PMX53, invented by Taylor and Woodruff and developed first by Peptech and then Arana Therapeutics109. This agent, modelled on the C terminus of C5a, showed high specificity and nanomolar affinity for C5aR1 and worked across many species; this latter property enabled the testing of PMX53 in a broad sweep of rodent models of inflammatory disease, and in many of these models this peptide had good efficacy. A number of Phase I and IIa clinical trials with PMX53 were initiated in rheumatoid arthritis, osteoarthritis, psoriasis and AMD; the molecule proved safe, but all trials were terminated because of poor efficacy. One possible reason for these failures was the poor oral bioavailability and tissue penetrance of PMX53 (Ref. 110). A modified version of PMX53, PMX205, shows improvements in these parameters and may re-surface in future trials, perhaps in combination with other agents. An orally active small molecule that blocks C5aR1 has also been developed by ChemoCentryx; this agent, CCX-168, has completed Phase II studies in anti-neutrophil cytoplasmic antibody (ANCA) vasculitis, in which it was successful in enabling the reduction and elimination of high-dose corticosteroids and demonstrated improvement in renal health parameters. In 2014, CCX-168 was granted Orphan Drug status in both ANCA-associated vasculitides and aHUS. Emerging evidence for the role of C3a as a 'modulator' of the C5a-driven inflammatory response highlights the potential for C3aR agonism in acute neutrophil-driven pathologies such as ischaemia–reperfusion injury106,111.

An alternative approach, again by analogy to the chemokine and cytokine field, is the use of blockers — either antibodies or others — that bind to C3a or C5a and prevent receptor engagement. Cytokine-specific antibodies and decoy receptors have evolved into blockbuster drugs. InflaRx have developed a blocking mAb against C5a (IFX-1) that is currently in a Phase II trial for early sepsis and septic shock. Noxxon described a series of L-RNA aptamers (Spiegelmer; NOX-D19 to NOX-D21) that block C5a; these agents were effective in models of sepsis and transplant rejection112,113, but have not yet progressed to humans. All of the above suggests that pharmacological blockade of C3a and/or C5a activities might be relatively straightforward; however, such an approach will only work in those situations in which these molecules are the major drivers of pathology, such as in neutrophil-mediated disorders including ANCA-vasculitis, chronic obstructive pulmonary disease (COPD) and lupus.

Targeting C5. As described above, C5 is activated to create two moieties with distinct activities. The small fragment, C5a, is a powerful chemoattractant, and therapeutic blockade of its activities is described above. The larger fragment, C5b, forms the foundation of the MAC. Assembly of the MAC involves the non-enzymatic aggregation of the five terminal pathway components (C5b and C6–C9) into a pore that inserts into and through the cell membrane. C5b, while still attached to the convertase, binds sequentially to C6 and C7; the C5b67 complex is released from the convertase and binds to the membrane via a labile hydrophobic site. C8 and multiple copies of C9 are recruited in turn to build the pore. MAC activity is naturally regulated by inefficiencies built into the assembly system and by regulators policing the membrane to block complexes that reach the surface. The hydrophobic binding site in C5b67 is intrinsically unstable, decaying by interaction with water in a fraction of a second. Additionally, there are several proteins in the plasma that bind to the MAC to further restrict its capacity to bind to the membrane: clusterin and vitronectin (also known as S protein) are multifunctional proteins that, among their many roles, inhibit MAC assembly in this way, and C8 is an efficient inhibitor if it binds to C5b67 in the fluid phase. Once bound to the membrane, C5b67 is protected from these plasma inhibitors and recruits C8 and C9 to form the MAC. The last line of defence is the membrane regulator CD59, a small glycosylphosphatidylinositol (GPI)-anchored protein that binds to the forming complex and prevents the completion of the MAC pore.

Opportunities for therapeutic inhibition are legion. The first obvious target is the first MAC component, C5b. Cutting off the supply of C5b by targeting the C3 and C5 convertases will inhibit MAC production; alternatively, C5 itself can be targeted to render it resistant to cleavage, which is the mode of action of the C5 monoclonal eculizumab, the most successful complement-specific drug to date. The success of eculizumab (Box 2) has triggered interest in other ways of preventing C5 cleavage. Novartis have taken a C5-specific mAb, LFG316, into Phase II trials of intraocular therapy in advanced AMD (completed but unpublished) and, more recently, in choroiditis and panuveitis114. In August 2015, Novartis announced Phase I trials to assess the safety and efficacy of LFG316 in patients with PNH (NCT02534909). A recombinant human C5-specific mAb fragment, Mubodina (Adienne Pharma & Biotech), that prevents cleavage of C5 has undergone early-stage trials in aHUS and other renal diseases, although no development has been reported recently. Ornithodoros moubata complement inhibitor (OmCI), a small (16 kDa) lipocalin present in the saliva of soft ticks, is a potent C5 inhibitor that probably protects the tick from complement in its blood meal115. Recombinant OmCI (Coversin; Volution Immuno Pharmaceuticals) blocked C5 in numerous species and inhibited disease in rodent models116,117. The drug bound C5 at a site remote from the convertase cleavage site, suggesting that it functioned by either blocking the interaction of C5 with the convertase or preventing conformational change essential for cleavage. Coversin completed Phase I trials in 2014 and is in development for PNH and other MAC-driven diseases. SOBI002 is a C5-blocker that is based on Affibody technology and developed by Swedish Orphan Biovitrum. Inclusion of an albumin-binding motif extends the plasma half-life of SOBI002. This small (~12 kDa) protein, derived using phage display, avidly binds to C5 and prevents cleavage; the simple structure of SOBI002 makes synthesis easy and inexpensive. SOBI002 is currently in a Phase I trial, which was recently listed as terminated (NCT02083666). Various non-biologic approaches have also been developed and are either soon to be tested or already in clinical trials. Ophthotech have developed a C5-specific aptamer (ARC1905, also known as Zimura) that, when given intraocularly in a Phase IIa study in combination with an antibody targeting vascular endothelial growth factor (VEGF), improved outcomes in patients with wet AMD. The RNAi approach taken by Alnylam is described above; at the 2014 American Society of Haematology (ASH) meeting, Alnylam reported effective knockdown in non-human primates using ALN-CC5 (see Further information), and Phase I trials are now underway with PNH as the primary indication. Interim Phase I data demonstrate substantial knock-down of C5 in single-ascending-dose studies in humans (as announced in a recent Alnylam press release). RaPharma have developed a cyclic peptide that binds to C5 and prevents cleavage and activation; promising preclinical data were reported at ASH 2014 (see Further information), and Phase I studies are proposed for the near future. As with many of the C5-targeting agents reported here, the primary indication is likely to be PNH. The crowded 'landscape' in the C5 space (Fig. 3) clearly illustrates the activity generated by the success of eculizumab and the desire to block the activities of this key component in the cascade.

Figure 3: Progress of complement therapeutics towards clinical use.
figure 3

The figure graphically illustrates a current 'snapshot' of the different areas of complement that are being targeted and the progress of the drugs — both marketed and en route to approval. It is a rapidly moving field and, inevitably, some compounds will progress, others will fail and new drugs will emerge to take their positions in the landscape in the coming months and years. One certainty is that this already crowded field will become much more so in the near future. Among the monoclonal antibodies, TNT003 and TNT009 (True North Therapeutics) target complement fragment C1s; OMS721 (Omeros) targets MBL-associated serine protease 2 (MASP2); Lampalizumab (Roche/Genentech) targets Factor D (FD), Bikaciomab (Novelmed) targets FB; IFX-1 (InflaRx) targets C5a; eculizumab (Soliris; Alexion Pharmaceuticals), LFG316 (Novartis), ALXN1210 and ALXN550 (Alexion) all target C5. Regenesance are developing an antibody that targets C6, and Novelmed are developing an antibody (NM9401) against properdin. Among the protein biologics, Berinert (CSL Behring), Ruconest (Salix Pharmaceuticals) and Cinryze (Shire Pharmaceuticals) are all C1INH preparations that target C1; AMY-201 (Amyndas) is a mini FH; TT30 (ALXN1102; Alexion) is a CR2–FH hybrid; TP10 (CDX-1135; Celldex Therapeutics) is a soluble form of complement receptor type 1 (CR1); Coversin (Volution Immuno Pharmaceuticals) is a C5-binding protein; Mirococept is a targeted CR1 fragment developed by AdProTech and currently in clinical trials led by King's College London; and the affibody SOBI002 (Swedish Orphan Biovitrum) targets C5 (programme recently terminated). Small-molecule inhibitors include CCX-168 (ChemoCentryx) and DF2593A (Dompé Pharmaceutical), which target C5aR; FD inhibitors from Achillion and Novartis; and a properdin inhibitor from Novelmed. Among the peptide-based therapeutics, Cp40 (Amyndas), APL-1 and APL-2 (Apellis Pharmaceuticals) are compstatin derivatives targeting C3; and RA101348 (RaPharma) is a C5 blocking peptide. Nucleic acid-based drugs include ARC1905 (Zimura; Ophthotech), a C5 aptamer; ALN-CC5 (Alnylam Pharmaceuticals), a C5 RNA interference (RNAi) molecule; and the Spiegelmers NOX-D19 to NOX-D21 (Noxxon Pharma), targeting C5a. Regenesance are developing a C6 antisense molecule.

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Targeting the terminal pathway: beyond C5. Blocking C5 cleavage by any of the above strategies will stop the production of C5a as well as the MAC and this may have important consequences if continued long term. To specifically target the MAC, agents with activities downstream of C5 cleavage are needed. Agents that mimic the ability of the natural inhibitors clusterin and vitronectin (and C8) to compete for the membrane binding site on C5b67 might further reduce the efficiency of membrane attack, whereas mimics of CD59 that block the final steps of MAC assembly might be effective blockers of lytic pore formation. Soluble recombinant CD59 (sCD59) has been generated and shown to inhibit lysis in vitro; however, in vivo, sCD59 binds to plasma proteins, markedly reducing its inhibitory effects118. Recombinant CD59, attached to the same membrane-binding motif as in Mirococept, has been generated and shown to protect PNH erythrocytes from lysis ex vivo and ameliorate arthritis development in a rat model when administered intra-articularly at the time of disease induction119,120. Alternatively, any one of the terminal pathway components, each of which is essential for MAC formation, could be targeted. Regenesance BV has targeted C6 with both blocking C6-specific mAb and antisense approaches, and the latter was effective in animal models105. Regenesance also described a small-molecule inhibitor of C6 in development for Guillain–Barré syndrome, but further development was not reported. A recent publication describes a small molecule (aurin tricarboxylic acid) that inhibits MAC assembly by blocking C9 binding; the agent prevented the lysis of PNH erythrocytes by serum ex vivo121.

Localizing therapeutics to sites of pathology

Inhibition of systemic complement activity is a major concern with most anti-complement drugs as it renders the recipient susceptible to infection. Delivery of the drug specifically to the site of disease will not only reduce risk but also enhance efficacy. At its simplest, local delivery could mean direct administration to the site of disease — into the inflamed joint, eye or brain, for example. More interesting are the many modifications that have been made to ensure the homing of an agent where needed. Modification of sCR1 to favour its accumulation on inflamed endothelia (TP20) increased efficacy in models89, and addition of a membrane-binding tag to a truncated form of sCR1 (Mirococept) converted an inactive fragment into a viable therapeutic90,91. Similarly, a recombinant truncated FH comprising the minimal active site (short consensus repeats 1–4) is essentially inactive in vivo, but adding in the FH membrane-binding short consensus repeats creates a powerful inhibitor (AMY-201)79,80.

Localising anti-complement agents to sites of complement activation has attracted abundant attention. Homing of the drug not only avoids systemic inhibition but also enhances efficacy by concentrating the drug in one site and by placing active sites in the correct orientation to inactivate target enzymes. These approaches tend to focus on the homing of agents that have regulatory functions, such as decay acceleration or cofactor activity, rather than the blocking activities typical of antibodies. It is important that the drug that is delivered to the site is able to 'recycle' and release its target once inactivated, otherwise the target surface will rapidly become saturated with useless agent. More than 20 years ago, we described a prodrug approach whereby recombinant forms of natural regulators were generated as Fc fusion proteins in which regulatory activity was absent because of steric hindrance. Engineered enzyme-cleavage sites enabled the release of active agent by matrix metalloproteinases and related enzymes that are abundant at inflammatory sites122. Similar strategies have proved successful in anticancer therapy123.

Complement fragments bind covalently to tissues at sites of activation and provide a potential ligand for localizing an anti-complement drug where needed. A hybrid molecule comprising the rodent C3 convertase regulator CRRY coupled to the C3dg-binding short consensus repeats of CR2 provided proof-of-principle of this approach; the hybrid molecule localized to sites of pathology and was 10-fold more active compared to CRRY alone in mouse models of ischaemia–reperfusion124. The same group also described a CR2–FH hybrid protein that was effective in several murine models125; this provided the impetus to create a human CR2–FH hybrid, TT30, which is currently under development by Alexion for therapy of PNH (ALXN1102 and ALXN1103)22,126. Potential advantages of this agent over eculizumab include a reduced risk of infection, a lower dose requirement and protection of PNH erythrocytes from C3 deposition and the resultant extravascular haemolysis. Progress towards the clinic has been slow, perhaps because of commercial rather than scientific considerations. A Phase I clinical trial was initiated (NCT01335165), although no data have been reported.

Looking to the future

Although the many roles of complement in driving pathology in diverse diseases have long been recognized, the capacity to treat these diseases by controlling complement activation has lagged behind. Today, we boast just two anti-complement drugs that are being marketed, approved only in rare orphan diseases (HAE, PNH and aHUS), which is a rather disappointing outcome given the investments made by researchers and companies. However, the future looks much brighter; new therapeutics are entering the clinic, targeting different parts of the complement system, with different half-lives, different routes of administration and each with its own range of activities and side effects (Fig. 3). Importantly, although the emphasis on orphan applications was pivotal in getting the first agents to the market, many of the new agents target common acute and chronic inflammatory diseases, for example AMD, arthritis and cardiovascular disease.

Hurdles to the commercialization of complement therapeutics still exist, including cost, routes of administration and infection risk. Short-duration complement inhibition, particularly in a controlled clinical environment, carries the least risk, and anti-complement drugs will probably first become mainstream for acute indications, such as reperfusion injuries. For chronic conditions, cost, ease of administration and safety may be substantial issues. Cost will become less of an issue as increased competition (the emergence of small-molecule inhibitors) and increased use should, theoretically, bring prices down rapidly. The experience of chronic therapy with agents that block complement is still relatively short-term, and it is possible that the elimination of molecules, such as C5a, may affect other activities of this multi-functional molecule, such as modulation of adaptive immunity, in the long term106. However, eculizumab was approved for use in PNH in 2007 and for aHUS in 2011 and, although the long-term consequences of C5 blockade remain uncertain, it is encouraging that after almost a decade of use no substantial adverse effects have been noted in carefully monitored and compliant recipients.

Frequent intravenous administration is not clinically acceptable for chronic use, and so agents will need either very long half-lives or to be delivered by routes not requiring medical intervention (for example, subcutaneous or oral). It may be that a therapy could be titrated to give partial inhibition of complement, sufficient to inhibit disease but sparing key protective functions. Alternatively, a drug may be engineered to deliver complement inhibition where it is needed at the site of disease. Inevitably, different diseases will require different approaches to complement inhibition — some may be entirely MAC-driven, others C5a-dependent, and most involve a complicated interplay between the various complement products and other inflammatory mediators.

Selecting the right agent or agents will be crucial to success in trials, and here a comprehensive understanding of the mechanisms by which complement drives pathology in the target disease is a sine qua non. Biomarkers that reflect complement activation in fluids and tissues can inform the selection of the right drug for the right patient, demonstrating engagement of the target and efficacy of the drug, and monitoring response to therapy. A lack of good biomarkers often handicaps early stage trials and simple readouts of effectiveness are crucial. In some cases this is obvious; for example, blockade of MAC formation can be demonstrated in serum through simple haemolytic assays. When activation fragments such as C3a, C5a, C4d, Bb and terminal complement complex (TCC) are present in the disease their levels can be monitored to demonstrate response to therapy and confirm target engagement. In some diseases, such as PNH, there is an obvious clinical readout of target engagement — eculizumab treatment prevents intravascular haemolysis in PNH. However, for diseases restricted to specific sites, for example, the retina, central nervous system or kidney glomerulus, plasma complement biomarkers may not reflect the response to therapy and are poor tools for assessing target engagement. There is thus a need for effective, non-invasive methods to detect complement activation and deposition within affected tissues without resorting to biopsy. Methods for imaging complement activation in vivo may fill this need; agents comprising the C3-binding domain of CR2 have been used to detect C3 fragments deposited in the kidney by magnetic resonance imaging in mouse models127. Antibodies against C3dg have also been developed as imaging agents and shown to identify retinal lesions in a mouse model of AMD128. Further development of the capacity to image complement activation in vivo will inform the conduct of future clinical trials by providing measurable outcomes and early proof of target engagement.