FIGURE 1 Key considerations when performing gene-expression profiling using microarray analysis.
From the following article:
Technology Insight: tuning into the genetic orchestra using microarrays—limitations of DNA microarrays in clinical practice
Ambreen Abdullah-Sayani, Jolien M Bueno-de-Mesquita and Marc J van de Vijver
BACK TO ARTICLE
Figure 1. Key considerations when performing gene-expression profiling using microarray analysis.
The different steps in this process and their limitations are summarized. There are seven major steps in the development of a gene-expression signature using microarrays before it is ready for implementation as a test in clinical practice. The first step consists of defining the scientific question that should be answered by the output data. The accompanying hypothesis will help to construct a solid study design and to determine the type of samples and the applicable inclusion criteria (steps 2 and 3). Step 4 includes the actual microarray experiment by which the gene-expression data are obtained. The statistical analysis of the data generated in the experiment and the construction of the actual gene-expression profile is addressed in step 5. Step 6 includes the independent validation of the results, which is indispensable for the transition to step 7, the clinical implementation of the gene-expression profile. Figure modified with permission from reference 21 © (2002) Nature Publishing Group.21
