FIGURE 4 Lymph-node biopsy and staining for vascular endothelial cells with CD34 and double immunofluorescent staining of COX2 in the plasma cells.
From the following article:
COX2-related multicentric mixed-type Castleman's disease in a young man
Jie Liu, Shuang Han, Jie Ding, Kaichun Wu, Jiyan Miao and Daiming Fan
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Figure 4. Lymph-node biopsy and staining for vascular endothelial cells with CD34 and double immunofluorescent staining of COX2 in the plasma cells.
(A) Hematoxylin and eosin staining of the lymph node showing blood vessel and plasma cell proliferation among the follicles. Magnification 
200. (B) Immunohistochemical staining of the plasma cells with an antiplasma cell antibody. Magnification 250. (C) Immunohistochemical analysis of the blood vessels by anti-CD34 staining (DAKO, Glostrup, Denmark). Magnification 200. (D) Paraffin-embedded tissue showing strong immunohistochemical staining of COX2 with an anti-COX2 polyclonal antibody (Cayman, Ann Arbor, MI). Magnification 200. (E–G) Confocal laser-scanning microscopy showing plasma cells expressing COX2 (E, COX2 staining with red channel; F, plasmacytes highlighted with the green channel; G, co-staining of COX2 and plasma cells [red plus green channel], primary and secondary antibodies as in Figure 2). Magnification 300. COX2, cyclooxygenase 2.
