FIGURE 2 Bone-marrow biopsy revealing heteromorphic plasmacyte aggregates and double immunofluorescent staining of COX2 in the plasma cells.
From the following article:
COX2-related multicentric mixed-type Castleman's disease in a young man
Jie Liu, Shuang Han, Jie Ding, Kaichun Wu, Jiyan Miao and Daiming Fan
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Figure 2. Bone-marrow biopsy revealing heteromorphic plasmacyte aggregates and double immunofluorescent staining of COX2 in the plasma cells.
(A) Plastic-embedded bone marrow (hematoxylin–Giemsa–eosin staining) showing a large area of mega-plasmacyte aggregation, with loose chromatin and visible nuclei (arrow). Magnification 
400. (B) Paraffin-embedded tissue showing plasmacytes highlighted by immunohistochemical staining with an antiplasmacyte antibody. Magnification 200. (C) Paraffin-embedded tissue showing strong immunohistochemical staining of COX2 with an anti-COX2 polyclonal antibody (Cayman, Ann Arbor, MI). Magnification 200. (D–F) Confocal laser-scanning microscopy of paraffin-embedded tissue showing strong immunofluorescent staining of COX2 with an anti-COX2 polyclonal antibody (D, red-channel, tetraethyl rhodamine isothiocyanate-labeled secondary antibody), plasmacytes highlighted by immunofluorescent staining with antiplasmacyte monoclonal antibody (E, green channel, fluorescein isothiocyanate-labeled secondary antibody), and double immunofluorescent staining showing plasma cells expressing COX2 (F, red plus green channel). Magnification 400. COX2, cyclooxygenase 2.
