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Tumorigenesis

Keeping a good thing down

Nature Reviews Cancer volume 8, pages 8–9 (2008)Cite this article

Using two models of MYC-induced tumorigenesis — human P493-6 B cells that are immortalized with Epstein–Barr virus and express a tetracycline-regulated form of MYC, and lymphomas formed by mouse p53-null bone-marrow cells that are infected with a MYC–oestrogen receptor (ER) retroviral construct, in which the MYC protein is regulated by tamoxifen — the authors analysed the fate of miR expression in conditions of high and low MYC activity using custom-built miR microarrays from human and mouse. Surprisingly, most miRs were downregulated in both models, with only the miR-17–92 cluster being consistently upregulated.

To further confirm these findings the authors analysed miR expression in P493-6 cells and MYC–ER tumours under conditions of high and low MYC expression using RNA blots. Because many of the miRs are encoded by several related transcripts, the authors developed stringent conditions in order to detect individual family members. In addition, they analysed expression levels of miRs in human tumours using previously published microarray profiles from MYC-expressing Burkitt lymphoma cells. These experiments consistently showed miR repression in response to MYC.

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ORIGINAL RESEARCH PAPER

  1. Chang, T.-C. et al. Widespread microRNA repression by Myc contributes to tumorigenesis. Nature Genet. 9 Dec 2007 (doi 10.1038/ng.2007.30)

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Authors
  1. Nicola McCarthy
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McCarthy, N. Keeping a good thing down. Nat Rev Cancer 8, 8–9 (2008). https://doi.org/10.1038/nrc2306

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  • DOI: https://doi.org/10.1038/nrc2306

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