Perspectives

Nature Reviews Cancer 6, 146-155 (February 2006) | doi:10.1038/nrc1804

Focus on: Biomarkers

InnovationMapping normal and cancer cell signalling networks: towards single-cell proteomics

Jonathan M. Irish1, Nikesh Kotecha2 & Garry P. Nolan3  About the authors

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Oncogenesis and tumour progression are supported by alterations in cell signalling. Using flow cytometry, it is now possible to track and analyse signalling events in individual cancer cells. Data from this type of analysis can be used to create a network map of signalling in each cell and to link specific signalling profiles with clinical outcomes. This form of 'single-cell proteomics' can identify pathways that are activated in therapy-resistant cells and can provide biomarkers for cancer diagnosis and for determining patient prognosis.

Author affiliations

  1. Jonathan M. Irish is at the Department of Medicine, Oncology Division, Stanford University, Stanford, California 94305, USA.
  2. Nikesh Kotecha is at the Department of Biomedical Informatics, Stanford University, Stanford, California 94305, USA.
  3. Garry P. Nolan is at the Baxter Laboratory for Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University, Stanford, California 94305, USA.

Correspondence to: Garry P. Nolan3 Email: gnolan@stanford.edu

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