Stem Cell series

Featured Content

• Differentiation of mouse embryonic stem cells to insulin-producing cells

  Insa S Schroeder et al.

  This protocol from the laboratory of Anna Wobus describes how to form embryoid bodies from mouse embryonic stem cells followed by the induction of progenitors to pancreatic cells. After approximately 33 days in culture the differentiated cells form islet-like clusters that respond to glucose with the release of insulin.

• Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines

  Alejandro Soto-Gutiérrez et al.

  In this protocol mouse embryonic stem cells are directed to differentiate into hepatocyte-like cells. The resulting cells express the hepatocyte specific genes albumin and CYP7a1.

• Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos

  Guy S Eakin & Anna-Katerina Hadjantonakis

  Mouse chimeras are produced in this protocol using either diploid or tetraploid morula. Tetraploid cells are formed by the electrofusion of day 1.5 post coitum embryos whereas diploid cells are collected from 8–cell embryos. The cells are then aggregated with embryonic stem cells to form chimeras.

• Evaluation of purified hematopoietic stem cells from umbilical cord blood

  Christopher Y Park, Ravindra Majeti & Irving L Weissman

  Human hematopoietic stem cells are isolated from umbilical cord blood in this protocol. Cells that are Lin-CD34+CD38-CD90 are transplanted into laboratory animals and long and short term engraftment is measured.

• Adult mouse hematopoietic stem cells: purification and single-cell assays

  Hideo Ema et al.

  This protocol isolates mouse hematopoietic stem cells based on their surface marker expression. CD34^−/lowc-Kit⁺Sca-1⁺lineage marker⁻ cells are isolated using flow assisted cell sorting and then used in single cell assays to measure their multilineage repopulating activity.

Protocols Archive

• Technology: Isolation and Preparation

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Generation of induced pluripotent stem cells from neural stem cells

    Published online: 17 September 2009 | doi:10.1038/nprot.2009.173

    Jeong Beom Kim, Holm Zaehres, Marcos J Araúzo-Bravo & Hans R Schöler

    • Abstract
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  • Isolation and cultivation of stem cells from adult mouse testes

    Published online: 15 January 2009 | doi:10.1038/nprot.2008.242

    Kaomei Guan, Frieder Wolf, Alexander Becker, Wolfgang Engel, Karim Nayernia & Gerd Hasenfuss

    • Abstract
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    - This protocol was used in a related article published in Nature

  • A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

    Published online: 8 January 2009 | doi:10.1038/nprot.2008.221

    Masoud Soleimani & Samad Nadri

    • Abstract
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    - This protocol was used in a related article published in Int. J. Dev. Biol.

  • Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique

    Published online: 4 September 2008 | doi:10.1038/nprot.2008.142

    Burhan Gharaibeh, Aiping Lu, Jessica Tebbets, Bo Zheng, Joe Feduska, Mihaela Crisan, Bruno Péault, James Cummins & Johnny Huard

    • Abstract
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  • Generation of human-induced pluripotent stem cells

    Published online: 26 June 2008 | Corrected online: 4 September 2008 | doi:10.1038/nprot.2008.92

    In-Hyun Park, Paul H Lerou, Rui Zhao, Hongguang Huo & George Q Daley

    • Abstract
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    - This protocol was used in a related article published in Nature Biotechnology

  • Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos

    Published online: 1 May 2008 | doi:10.1038/nprot.2008.60

    Paul H Lerou, Akiko Yabuuchi, Hongguang Huo, Justine D Miller, Leah F Boyer, Thorsten M Schlaeger & George Q Daley

    • Abstract
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    - This protocol was used in a related article published in Nature Biotechnology

  • Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro

    Published online: 29 May 2008 | doi:10.1038/nprot.2008.69

    Colin McGuckin, Marcin Jurga, Hamad Ali, Marko Strbad & Nicolas Forraz

    • Abstract
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  • Induction of pluripotent stem cells from fibroblast cultures

    Published online: 29 November 2007 | doi:10.1038/nprot.2007.418

    Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa & Shinya Yamanaka

    • Abstract
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    - This protocol was used in a related article published in Cell

  • Derivation of human embryonic stem cells from single blastomeres

    Published online: 9 August 2007 | doi:10.1038/nprot.2007.274

    Irina Klimanskaya, Young Chung, Sandy Becker, Shi-Jiang Lu & Robert Lanza

    • Abstract
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    - This protocol was used in a related article published in Nature

  • Adult mouse hematopoietic stem cells: purification and single-cell assays

    Published online: 25 January 2007 | doi:10.1038/nprot.2006.447

    Hideo Ema, Yohei Morita, Satoshi Yamazaki, Azusa Matsubara, Jun Seita, Yuko Tadokoro, Hiroyoshi Kondo, Hina Takano & Hiromitsu Nakauchi

    • Abstract
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    - This protocol was used in a related article published in Science

  • Derivation of mouse embryonic stem cells

    Published online: 30 November 2006 | doi:10.1038/nprot.2006.355

    Vítčzslav Bryja, Sonia Bonilla & Ernest Arenas

    • Abstract
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• Technology: Culturing and imaging cells

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Long Term Non-Invasive Imaging of Embryonic Stem Cells Using Reporter Genes

    Published online: 23 July 2009 | doi:10.1038/nprot.2009.100.

    Ning Sun, Andrew Lee, Joseph C. Wu

    • Abstract
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  • In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood

    Published online: 20 November 2008 | doi:10.1038/nprot.2008.194

    Christopher Y Park, Ravindra Majeti & Irving L Weissman

    • Abstract
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    - This protocol was used in a related article published in Cell Stem Cell

  • Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification

    Published online: 14 August 2008 | doi:10.1038/nprot.2008.140

    Stefan R Braam, Chris Denning, Elena Matsa, Lorraine E Young, Robert Passier & Christine L Mummery

    • Abstract
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    - This protocol was used in a related article published in Nature Methods

  • Optimized mouse ES cell culture system by suspension growth in a fully defined medium

    Published online: 22 May 2008 | doi:10.1038/nprot.2008.65

    Michael Andäng, Annalena Moliner, Claudia A Doege, Carlos F Ibañez & Patrik Ernfors

    • Abstract
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  • A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies

    Published online: 10 April 2008 | doi:10.1038/nprot.2008.42

    Elizabeth S Ng, Richard Davis, Edouard G Stanley & Andrew G Elefanty

    • Abstract
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    - This protocol was used in a related article published in Blood

  • A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells

    Published online: 5 April 2007 | doi:10.1038/nprot.2007.104

    Sacri R Ferrón, Celia Andreu-Agulló, Helena Mira, Pilar Sánchez, M Ángeles Marqués-Torrejón & Isabel Fariñas

    • Abstract
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  • In vitro generation of germ cells from murine embryonic stem cells

    Published online: 30 November 2006 | doi:10.1038/nprot.2006.303

    Jason A West, In-Hyun Park, George Q Daley & Niels Geijsen

    • Abstract
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    - This protocol was used in a related article published in Nature

  • Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos

    Published online: 7 September 2006 | doi:10.1038/nprot.2006.173

    Guy S Eakin & Anna-Katerina Hadjantonakis

    • Abstract
    •  Full Text
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• Differentiation of stem cells to specific lineages

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Differentiation of human oligodendrocytes from pluripotent stem cells

    Published online: 15 October 2009 | doi:10.1038/nprot.2009.186

    Bao-Yang Hu, Zhong-Wei Du & Su-Chun Zhang

    • Abstract
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  • Generation of cortical neurons from mouse embryonic stem cells

    Published online: 17 September 2009 | doi:10.1038/nprot.2009.157

    Nicolas Gaspard, Tristan Bouschet, Adèle Herpoel, Gilles Naeije, Jelle van den Ameele & Pierre Vanderhaeghen

    • Abstract
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  • Differentiation of spinal motor neurons from pluripotent human stem cells

    Published online: 20 August 2009 | doi:10.1038/nprot.2009.127.

    Bao-Yang Hu & Su-Chun Zhang

    • Abstract
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  • Stepwise differentiation of pluripotent stem cells into retinal cells

    Published online: 7 May 2009 | doi:10.1038/nprot.2009.51

    Fumitaka Osakada, Hanako Ikeda, Yoshiki Sasai & Masayo Takahashi

    • Abstract
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  • Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale

    Published online: 13 November 2008 | doi:10.1038/nprot.2008.188

    Myung-Soo Cho, Dong-Youn Hwang & Dong-Wook Kim

    • Abstract
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    - This protocol was used in a related article published in Proc. Natl. Acad. Sci. USA

  • Cardiac commitment of primate embryonic stem cells

    Published online: 7 August 2008 | doi:10.1038/nprot.2008.116

    Julia Leschik, Sonia Stefanovic, Benjamin Brinon & Michel Pucéat

    • Abstract
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    - This protocol was used in a related article published in Stem Cells

  • Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells

    Published online: 8 May 2008 | doi:10.1038/nprot.2008.55

    Andrew Chojnacki & Samuel Weiss

    • Abstract
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    - This protocol was used in a related article published in Science

  • Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells

    Published online: 26 April 2007 | doi:10.1038/nprot.2007.147

    Miriam Bibel, Jens Richter, Emmanuel Lacroix & Yves-Alain Barde

    • Abstract
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  • Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines

    Published online: 8 March 2007 | doi:10.1038/nprot.2007.18

    Alejandro Soto-Gutiérrez, Nalu Navarro-Álvarez, Debiao Zhao, Jorge D Rivas-Carrillo, Jane Lebkowski, Noriaki Tanaka, Ira J Fox & Naoya Kobayashi

    • Abstract
    •  Full Text
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    - This protocol was used in a related article published in Nature Biotechnology

  • In vitro generation of germ cells from murine embryonic stem cells

    Published online: 30 November 2006 | doi:10.1038/nprot.2006.303

    Jason A West, In-Hyun Park, George Q Daley & Niels Geijsen

    • Abstract
    •  Full Text
    •  PDF

    - This protocol was used in a related article published in Nature

  • Differentiation of mouse embryonic stem cells to insulin-producing cells

    Published online: 29 June 2006 | Corrected online: 5 October 2006 | doi:10.1038/nprot.2006.71

    Insa S Schroeder, Alexandra Rolletschek, Przemyslaw Blyszczuk, Gabriela Kania & Anna M Wobus

    • Abstract
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    - This protocol was used in a related article published in Blood

  • From The Protocols Network: Uploaded directly by recent Nature authors & other researchers
    
    

    Generation of neurospheres from embryonic stem cells for investigating the temporal specification of neural stem/progenitor cells

    Author(s): Hayato Naka, Shiho Nakamura, Takuya Shimazaki and Hideyuki Okano

    Lab/Group: Okano Lab (Keio)

    DOI: 10.1038/nprot.2008.168

    •  Full Text

    - This protocol was used in a related article published in Nature Neuroscience

  • Isolation of Neural Crest Cells Derived From Human Embryonic Stem Cells

    Author(s): Gabsang Lee, Hyesoo Kim and Lorenz Studer

    Lab/Group: Studer Lab (SKI)

    DOI: 10.1038/nprot.2009.49

    •  Full Text

    - This protocol was used in a related article published in Nature Biotechnology

• Stem cells and progenitor cells isolated from adult tissues

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Isolation and cultivation of stem cells from adult mouse testes

    Published online: 15 January 2009 | doi:10.1038/nprot.2008.242

    Kaomei Guan, Frieder Wolf, Alexander Becker, Wolfgang Engel, Karim Nayernia & Gerd Hasenfuss

    • Abstract
    •  Full Text
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    - This protocol was used in a related article published in Nature

  • Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology

    Published online: 5 February 2009 | doi:10.1038/nprot.2008.229

    Anke M Smits, Patrick van Vliet, Corina H Metz, Tom Korfage, Joost PG Sluijter, Pieter A Doevendans & Marie-José Goumans

    • Abstract
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  • A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

    Published online: 8 January 2009 | doi:10.1038/nprot.2008.221

    Masoud Soleimani & Samad Nadri

    • Abstract
    •  Full Text
    •  PDF

    - This protocol was used in a related article published in Int. J. Dev. Biol.

  • Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique

    Published online: 4 September 2008 | doi:10.1038/nprot.2008.142

    Burhan Gharaibeh, Aiping Lu, Jessica Tebbets, Bo Zheng, Joe Feduska, Mihaela Crisan, Bruno Péault, James Cummins & Johnny Huard

    • Abstract
    •  Full Text
    •  PDF

  • Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells

    Published online: 8 May 2008 | doi:10.1038/nprot.2008.55

    Andrew Chojnacki & Samuel Weiss

    • Abstract
    •  Full Text
    •  PDF

    - This protocol was used in a related article published in Science

  • Retrovirally mediated telomerase immortalization of neural progenitor cells

    Published online: 1 November 2007 | doi:10.1038/nprot.2007.402

    Neeta S Roy, Devin Chandler-Militello, Gang Lu, Su Wang & Steven A Goldman

    • Abstract
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    - This protocol was used in a related article published in Nature Biotechnology

  • Establishment of 3D organotypic cultures using human neonatal epidermal cells

    Published online: 8 February 2007 | doi:10.1038/nprot.2006.448

    Pradnya Gangatirkar, Sophie Paquet-Fifield, Amy Li, Ralph Rossi & Pritinder Kaur

    • Abstract
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  • Adult mouse hematopoietic stem cells: purification and single-cell assays

    Published online: 25 January 2007 | doi:10.1038/nprot.2006.447

    Hideo Ema, Yohei Morita, Satoshi Yamazaki, Azusa Matsubara, Jun Seita, Yuko Tadokoro, Hiroyoshi Kondo, Hina Takano & Hiromitsu Nakauchi

    • Abstract
    •  Full Text
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    - This protocol was used in a related article published in Science

  • Isolation and culture of rat and mouse oligodendrocyte precursor cells

    Published online: 26 April 2007 | doi:10.1038/nprot.2007.149

    Ying Chen, Veerakumar Balasubramaniyan, Jie Peng, Edward C Hurlock, Michelle Tallquist, Jianrong Li & Q Richard Lu

    • Abstract
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  • Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny

    Published online: 25 January 2007 | doi:10.1038/nprot.2006.422

    Jeffrey A Biernaskie, Ian A McKenzie, Jean G Toma & Freda D Miller

    • Abstract
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    - This protocol was used in a related article published in Nature Cell Biology

  • From The Protocols Network: Uploaded directly by recent Nature authors & other researchers
    
    

    Generation of stem/progenitor cultures from mouse lungs

    Author(s): Juan-Jose Ventura

    Lab/Group: Nebreda Lab

    DOI: 10.1038/nprot.2007.216

    •  Full Text

    - This protocol was used in a related article published in Nature Genetics

  • Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

    Author(s): Laura Pacey KK, Shelley Stead, Jacqueline Gleave A, Kasia Tomczyk and Laurie Doering C

    Lab/Group: Department of Pathology and Molecular Medicine, McMaster University

    DOI: 10.1038/nprot.2006.215

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  • Cell staining for sorting of hematopoietic stem cells (HSC) and myeloid progenitors and isolating RNA from sorted cells

    Author(s): Hideyo Hirai, Pu Zhang, Tajhal Dayaram, Christopher Hetherington, Shin-ichi Mizuno...

    Lab/Group: Hurles Lab (Sanger Institute)

    DOI: 10.1038/nprot.2006.139

    •  Full Text

    - This protocol was used in a related article published in Nature Immunology

• Tissue regeneration

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Electrophysiological evaluation of engrafted stem cell-derived neurons

    Published online: 21 June 2007 | doi:10.1038/nprot.2007.230

    Thoralf Opitz, Björn Scheffler, Barbara Steinfarz, Tanja Schmandt & Oliver Brüstle

    • Abstract
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  • A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction

    Published online: 9 November 2006 | doi:10.1038/nprot.2006.188

    Ngan F Huang, Richard E Sievers, Jennifer S Park, Qizhi Fang, Song Li & Randall J Lee

    • Abstract
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• Stem cells and molecular biology

  • From Nature Protocols: Peer reviewed & editorially enhanced
    
    

    Attachment of stem cells to scaffold particles for intracerebral transplantation

    Published online: 17 September 2009 | doi:10.1038/nprot.2009.156

    Ellen Bible et al.

    • Abstract
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  • Long Term Non-Invasive Imaging of Embryonic Stem Cells Using Reporter Genes

    Published online: 23 July 2009 | doi:10.1038/nprot.2009.100

    Ning Sun, Andrew Lee, Joseph C. Wu

    • Abstract
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  • Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells

    Published online: 26 March 2009 | doi:10.1038/nprot.2009.23

    Jonghwan Kim, Alan B Cantor, Stuart H Orkin & Jianlong Wang

    • Abstract
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    - This protocol was used in a related article published in Nature

  • A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis

    Published online: 11 September 2008 | doi:10.1038/nprot.2008.146

    Richard P Davis, Magdaline Costa, Catarina Grandela, Andrew M Holland, Tanya Hatzistavrou, Suzanne J Micallef, Xueling Li, Adam L Goulburn, Lisa Azzola, Andrew G Elefanty & Edouard G Stanley

    • Abstract
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  • An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies

    Published online: 26 June 2008 | doi:10.1038/nprot.2008.103

    Denis Piette, Marijke Hendrickx, Erik Willems, Caroline R Kemp & Luc Leyns

    • Abstract
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    - This protocol was used in a related article published in Developmental Dynamics

  • A method for genetic modification of human embryonic stem cells using electroporation

    Published online: 5 April 2007 | doi:10.1038/nprot.2007.105

    Magdaline Costa, Mirella Dottori, Koula Sourris, Pegah Jamshidi, Tanya Hatzistavrou, Richard Davis, Lisa Azzola, Steven Jackson, Sue Mei Lim, Martin Pera, Andrew G Elefanty & Edouard G Stanley

    • Abstract
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    - This protocol was used in a related article published in Nature Methods

  • From The Protocols Network: Uploaded directly by recent Nature authors & other researchers
    
    

    Two-Photon Microscopy and Spectral Detection for Ex Vivo Imaging of Individual Stem Cells

    DOI: 10.1038/nprot.2009.48

    Author(s): Winfried Wiegraebe, Yucai Xie, Tong Yin, Danny Stark, Katherine Perko, Richard Alexander, Joel Sc...

    Lab/Group: Linheng Li Lab, Advanced Instrumentation & Physics Facility

    •  Full Text

    This protocols was used in a related article published in Nature

  • Cell staining for sorting of hematopoietic stem cells (HSC) and myeloid progenitors and isolating RNA from sorted cells

    DOI: 10.1038/nprot.2006.139

    Author(s): Hideyo Hirai, Pu Zhang, Tajhal Dayaram, Christopher Hetherington, Shin-ichi Mizuno...

    Lab/Group: Hurles Lab (Sanger Institute)

    •  Full Text

    - This protocol was used in a related article published in Nature Immunology

Movies

• Technology: Isolation and cultivation of stem cells from adult mouse testes

  The successful isolation and cultivation of spermatogonial stem cells (SSCs) as well as induction of SSCs into pluripotent stem cells will allow us to study their biological characteristics and their applications in therapeutic approaches.

• Technology: Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos

  The production of mouse chimeras is a common step in the establishment of genetically modified animal strains. Chimeras also provide a powerful experimental tool for following cell behavior during both prenatal and postnatal development.

Other content of interest to stem cell researchers on nature.com

• Nature Stem Cell Reports
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