Volume 9 Issue 5, May 2014

Maximum projection views of the same falsely colored live C. elegans embryo, labeled with a GFP cell membrane marker and imaged using Bessel beam structured illumination microscopy. Image adapted from the protocol by Gao et al. doi:10.1038/nprot.2014.087. Cover design by Jamel Wooten.

Protocol

Targeted sequencing for gene discovery and quantification using RNA CaptureSeq

Tim R Mercer
Michael B Clark
John S Mattick
Protocol 04 Apr 2014
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Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants

Benjamin Dugdale
Cara L Mortimer
James L Dale
Protocol 04 Apr 2014
Single-cell axotomy of cultured hippocampal neurons integrated in neuronal circuits

Susana Gomis-Rüth
Michael Stiess
Frank Bradke
Protocol 04 Apr 2014
Obtaining genomes from uncultivated environmental microorganisms using FACS–based single-cell genomics

Christian Rinke
Janey Lee
Tanja Woyke
Protocol 10 Apr 2014
Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy

Audrey Beaussart
Sofiane El-Kirat-Chatel
Yves F Dufrêne
Protocol 10 Apr 2014
Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX

Mikkel Schubert
Luca Ermini
Ludovic Orlando
Protocol 10 Apr 2014
3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

Liang Gao
Lin Shao
Eric Betzig

Collection:

International Year of Light 2015
Protocol 10 Apr 2014
Detecting apoptosis of leukocytes in mouse lymph nodes

Laura Gómez-Cabañas
Cristina Delgado-Martín
José Luis Rodríguez-Fernández
Protocol 17 Apr 2014
Multiparametric high-resolution imaging of native proteins by force-distance curve–based AFM

Moritz Pfreundschuh
David Martinez-Martin
Daniel J Muller
Protocol 17 Apr 2014
Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry

Sarela García-Santamarina
Susanna Boronat
Elena Hidalgo
Protocol 17 Apr 2014
Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence probe

Xiaoming Zhou
Debin Zhu
Da Xing
Protocol 17 Apr 2014
In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors

Sergio Gonzalez
Ruani N Fernando
Nicolas Tricaud
Protocol 24 Apr 2014
Microinjection of membrane-impermeable molecules into single neural stem cells in brain tissue

Fong Kuan Wong
Christiane Haffner
Elena Taverna
Protocol 24 Apr 2014
Scalable and chromatography-free synthesis of 2-(2-formylalkyl)arenecarboxylic acid derivatives through the supramolecularly controlled hydroformylation of vinylarene-2-carboxylic acids

This protocol describes how to prepare 2-(2-formylalkyl)-arenecarboxylic acid derivatives, common building blocks for the synthesis of various valuable chemicals (e.g., anti-obesity and Alzheimer's disease treatment pharmaceuticals), by using the fully regioselective hydroformylation of vinyl arene derivatives.

Paweł Dydio
Joost N H Reek
Protocol 24 Apr 2014
Quality control and conduct of genome-wide association meta-analyses

A protocol providing guidelines on the organizational aspects of genome-wide association meta-analyses and to implement quality control at the study file level, the meta-level across studies, and the meta-analysis output level.

Thomas W Winkler
Felix R Day
Ruth J F Loos

Collection:

Statistics for Biologists
Protocol 24 Apr 2014

Volume 9 Issue 5, May 2014

Protocol

Targeted sequencing for gene discovery and quantification using RNA CaptureSeq

Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants

Single-cell axotomy of cultured hippocampal neurons integrated in neuronal circuits

Obtaining genomes from uncultivated environmental microorganisms using FACS–based single-cell genomics

Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy

Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX

3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

Detecting apoptosis of leukocytes in mouse lymph nodes

Multiparametric high-resolution imaging of native proteins by force-distance curve–based AFM

Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry

Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence probe

In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors

Microinjection of membrane-impermeable molecules into single neural stem cells in brain tissue

Scalable and chromatography-free synthesis of 2-(2-formylalkyl)arenecarboxylic acid derivatives through the supramolecularly controlled hydroformylation of vinylarene-2-carboxylic acids

Quality control and conduct of genome-wide association meta-analyses

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