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In vivo multiphoton image of an inflammatory lesion in the spinal cord of a transgenic mouse with genetically labeled axons counterstained with MitoTracker Red (magenta) and POPO-1 iodide (cyan) to visualize myelin and nuclei, respectively. Inflammation is indicated by infiltrating cells, myelin and axonal damage. Image taken from the protocol by Romanelli et al. doi:10.1038/nprot.2013.022. Cover design by Jamel Wooten.