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Dual protein immunostaining and fluorescent in situ hybridization of Drosophila egg chambers. gurken mRNA (magenta) is localized to the posterior of the oocyte. The α-Spectrin protein (green) marks cell membranes. DAPI (blue) reveals the polyploid nurse-cell (large) and follicle-cell (small) nuclei. Image taken from the protocol by Zimmerman et al. doi:10.1038/nprot.2013.136. Cover design by Jamel Wooten.