RGB marking of 293T cells with increasing amounts of RGB vectors.
Influence of exposure time on pictures of RGB-marked cells.
RGB-marked 293T cells.
Theoretical approach to RGB marking.
Properties of fluorescent proteins used for RGB marking and appropriate filter sets.
Sample pipetting scheme for transfection of 293T cells.
Pipetting scheme for RGB marking of 293T cells.
Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- Kristoffer Weber,
- Michael Thomaschewski &
- Boris Fehse
Internal Medicine I, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
- Daniel Benten
K.W. and B.F. developed the idea of RGB marking. K.W. and M.T. produced LeGO vectors and performed and analyzed gene transfer experiments in different target cells. K.W. and B.F. designed the study and wrote the manuscript. D.B. and B.F. supervised experiments, analyzed and evaluated results. All authors read and approved the final version of the manuscript.
Competing financial interests
The authors declare no competing financial interests.