Volume 7 Issue 11, November 2012

Three-dimensional reconstruction of the entire mouse brain vasculature imaged by 3DISCO and shown in a color-coded map. The vasculature was labeled with lectin-FITC and imaged by ultramicroscopy after tissue clearing. Image taken from the protocol by Ertürk et al. doi: 10.1038/nprot.2012.119. Cover design by Jamel Wooten.

Protocol

Using multiplex single-base extension typing to screen for mutants defective in RNA editing

Mizuki Takenaka
Axel Brennicke
Protocol 04 Oct 2012
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One-step ¹⁸F-labeling of peptides for positron emission tomography imaging using the SiFA methodology

Carmen Wängler
Sabrina Niedermoser
Björn Wängler
Protocol 04 Oct 2012
Synthesis of [¹⁸F]SiFB: a prosthetic group for direct protein radiolabeling for application in positron emission tomography

Alexey P Kostikov
Joshua Chin
Ralf Schirrmacher
Protocol 04 Oct 2012
Protein labeling with the labeling precursor [¹⁸F]SiFA-SH for positron emission tomography

Björn Wängler
Alexey P Kostikov
Ralf Schirrmacher
Protocol 04 Oct 2012
Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos

Martin Zeeb
Jennifer Axnick
Eckhard Lammert
Protocol 11 Oct 2012
Three-dimensional imaging of solvent-cleared organs using 3DISCO

Ali Ertürk
Klaus Becker
Hans-Ulrich Dodt
Protocol 11 Oct 2012
Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue

Rossukon Kaewkhaw
Andy M Scutt
John W Haycock
Protocol 11 Oct 2012
Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse

Weixiang Guo
Natalie E Patzlaff
Xinyu Zhao
Protocol 18 Oct 2012
Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression

Sarah N Dowey
Xiaosong Huang
Linzhao Cheng
Protocol 18 Oct 2012
A scalable synthesis of 5,5′-dibromo-2,2′-bipyridine and its stepwise functionalization via Stille couplings

Daniel M D'Souza
David A Leigh
Steffen L Woltering
Protocol 18 Oct 2012
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions

Jeanette Beers
Daniel R Gulbranson
Guokai Chen
Protocol 25 Oct 2012
ZIC-cHILIC as a fractionation method for sensitive and powerful shotgun proteomics

Serena Di Palma
Shabaz Mohammed
Albert J R Heck
Protocol 25 Oct 2012
Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior

Robert S Fischer
Kenneth A Myers
Clare M Waterman
Protocol 25 Oct 2012

Volume 7 Issue 11, November 2012

Protocol

Using multiplex single-base extension typing to screen for mutants defective in RNA editing

One-step ¹⁸F-labeling of peptides for positron emission tomography imaging using the SiFA methodology

Synthesis of [¹⁸F]SiFB: a prosthetic group for direct protein radiolabeling for application in positron emission tomography

Protein labeling with the labeling precursor [¹⁸F]SiFA-SH for positron emission tomography

Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos

Three-dimensional imaging of solvent-cleared organs using 3DISCO

Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue

Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse

Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression

A scalable synthesis of 5,5′-dibromo-2,2′-bipyridine and its stepwise functionalization via Stille couplings

Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions

ZIC-cHILIC as a fractionation method for sensitive and powerful shotgun proteomics

Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior

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