Volume 6 Issue 2, February 2011

A confocal micrograph showing astroglia from the early postnatal mouse cerebral cortex in culture. The cells are immunostained for glial fibrillary acidic protein (GFAP, green) and with DAPI to show nuclei (blue). Image from the protocol by Heinrich et al. Cover design by Jamel Wooten.

Protocol

Basic statistical analysis in genetic case-control studies

Geraldine M Clarke
Carl A Anderson
Krina T Zondervan

Collection:

Statistics for Biologists
Protocol 03 Feb 2011
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Creating localized DNA double-strand breaks with microirradiation

Keiji Suzuki
Motohiro Yamauchi
Shunichi Yamashita
Protocol 13 Jan 2011
Rapid isolation of single malaria parasite–infected red blood cells by cell sorting

Jun Miao
Liwang Cui
Protocol 20 Jan 2011
Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics

Tamar Geiger
Jacek R Wisniewski
Matthias Mann
Protocol 01 Feb 2011
Application of isothermal titration calorimetry and column chromatography for identification of biomolecular targets

Xingding Zhou
R Manjunatha Kini
J Sivaraman
Protocol 20 Jan 2011
cDNA library generation from ribonucleoprotein particles

Mathieu Rederstorff
Alexander Hüttenhofer
Protocol 27 Jan 2011
Absolute quantification of protein and post-translational modification abundance with stable isotope–labeled synthetic peptides

Arminja N Kettenbach
John Rush
Scott A Gerber
Protocol 27 Jan 2011
Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity

Michael T Yang
Jianping Fu
Christopher S Chen
Protocol 27 Jan 2011
Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex

Christophe Heinrich
Sergio Gascón
Benedikt Berninger
Protocol 03 Feb 2011
High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope

Jessica S Blackburn
Sali Liu
David M Langenau
Protocol 03 Feb 2011
Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

Jiayuan Quan
Jingdong Tian
Protocol 03 Feb 2011

Volume 6 Issue 2, February 2011

Protocol

Basic statistical analysis in genetic case-control studies

Creating localized DNA double-strand breaks with microirradiation

Rapid isolation of single malaria parasite–infected red blood cells by cell sorting

Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics

Application of isothermal titration calorimetry and column chromatography for identification of biomolecular targets

cDNA library generation from ribonucleoprotein particles

Absolute quantification of protein and post-translational modification abundance with stable isotope–labeled synthetic peptides

Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity

Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex

High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

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