Volume 4 Issue 9, September 2009

Spinal motor neurons (labeled by HB9 in red), along with other neurons (labeled by β III-tubulin in green), are differentiated from human embryonic stem cells using the stepwise protocol (all cell nuclei shown in blue). Image is from the protocol by Hu and Zhang. Cover design by Jamel Wooten.

Protocol

Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase

F Peter Guengerich
Martha V Martin
Qian Cheng
Protocol 06 Aug 2009
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Chromatographic assays of drug oxidation by human cytochrome P450 3A4

Christal D Sohl
Qian Cheng
F Peter Guengerich
Protocol 06 Aug 2009
High-throughput fluorescence assay of cytochrome P450 3A4

Qian Cheng
Christal D Sohl
F Peter Guengerich
Protocol 06 Aug 2009
Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria

Juliane Neupert
Ralph Bock
Protocol 13 Aug 2009
m-TAG: a PCR-based genomic integration method to visualize the localization of specific endogenous mRNAs in vivo in yeast

Liora Haim-Vilmovsky
Jeffrey E Gerst
Protocol 13 Aug 2009
Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection

Yiorgos Apidianakis
Laurence G Rahme
Protocol 13 Aug 2009
Differentiation of spinal motor neurons from pluripotent human stem cells

Bao-Yang Hu
Su-Chun Zhang
Protocol 20 Aug 2009
Quantifying cellular interaction dynamics in 3D fluorescence microscopy data

Frederick Klauschen
Masaru Ishii
Martin Meier-Schellersheim
Protocol 20 Aug 2009
Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labeled glucose

Derek C Macallan
Becca Asquith
Peter C L Beverley
Protocol 20 Aug 2009
Mouse closed head injury model induced by a weight-drop device

Michael A Flierl
Philip F Stahel
Esther Shohami
Protocol 27 Aug 2009
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression

Xue-Ming Xu
Min-Hyuk Yoo
Dolph L Hatfield
Protocol 27 Aug 2009
Multi-stage chemical carcinogenesis in mouse skin: Fundamentals and applications

Erika L Abel
Joe M Angel
John DiGiovanni
Protocol 27 Aug 2009
Detection of protein SUMOylation in vivo

Michael H Tatham
Manuel S Rodriguez
Ronald T Hay
Protocol 03 Sept 2009
Preparation of carbon nanotube bioconjugates for biomedical applications

Zhuang Liu
Scott M Tabakman
Hongjie Dai
Protocol 03 Sept 2009
Expression, purification and use of recombinant annexin V for the detection of apoptotic cells

Susan E Logue
Mohamed Elgendy
Seamus J Martin
Protocol 03 Sept 2009

Volume 4 Issue 9, September 2009

Protocol

Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase

Chromatographic assays of drug oxidation by human cytochrome P450 3A4

High-throughput fluorescence assay of cytochrome P450 3A4

Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria

m-TAG: a PCR-based genomic integration method to visualize the localization of specific endogenous mRNAs in vivo in yeast

Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection

Differentiation of spinal motor neurons from pluripotent human stem cells

Quantifying cellular interaction dynamics in 3D fluorescence microscopy data

Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labeled glucose

Mouse closed head injury model induced by a weight-drop device

Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression

Multi-stage chemical carcinogenesis in mouse skin: Fundamentals and applications

Detection of protein SUMOylation in vivo

Preparation of carbon nanotube bioconjugates for biomedical applications

Expression, purification and use of recombinant annexin V for the detection of apoptotic cells

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