Supplementary information : Coupling surface plasmon resonance to mass spectrometry to discover novel protein-protein interactions : Nature Protocols

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Coupling surface plasmon resonance to mass spectrometry to discover novel protein–protein interactions

Alexandra Madeira, Elisabet Öhman, Anna Nilsson, Benita Sjögren, Per E Andrén & Per Svenningsson

Nature Protocols 4, 1023 - 1037 (2009) Published online: 11 June 2009

doi:10.1038/nprot.2009.84

Supplementary Figure 1 Program to immobilize a peptide on a carboxymethylated sensor chip.

This program consists of a sequence of commands controlling the immobilization experiment. A translation of these commands can be found in the procedure. Exclamation points allow adding some comments which are not included in the operation.

Supplementary Figure 1 Program to immobilize a peptide on a carboxymethylated sensor chip. - Download PowerPoint (128KB)

Supplementary Figure 2 Program to bind and recover proteins against a peptide on a carboxymethylated sensor chip.

This program consists of a sequence of commands controlling the recovery experiments in the instrument. A translation of these commands can be found in the procedure. Exclamation points allow adding some comments which are not included in the operation.

Supplementary Figure 2 Program to bind and recover proteins against a peptide on a carboxymethylated sensor chip. - Download PowerPoint (130KB)

Supplementary Figure 3 Program to bind and recover proteins against a peptide on a carboxymethylated chip in the surface prep unit.

This program consists of a sequence of commands controlling the recovery experiments in the surface prep unit. A translation of these commands can be found in the procedure. Exclamation points allow adding some comments which are not included in the operation.

Supplementary Figure 3 Program to bind and recover proteins against a peptide on a carboxymethylated chip in the surface prep unit. - Download PowerPoint (131KB)

Supplementary Figure 4 Mass spectra assigned to the peptide ASFTTFTVTK derived from Caveolin-1 (P41350).

The spectra contain the parent mass, charge and observed fragmentation pattern in the form of peak list. Monoisotopic mass of neutral peptide Mr (calc): 1101.57. Ions Score: 64; Expect: 1.7e-005. Matches (Bold Red): 12/86 fragment ions using 24 most intense peaks (modified and reproduced with permission from 5). All animal experiments are performed according to the local ethical committee at the Karolinska Institute (Application N282/06)

Supplementary Figure 4 Mass spectra assigned to the peptide ASFTTFTVTK derived from Caveolin-1 (P41350). - Download PowerPoint (91KB)

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