Supplementary information
From the following article
Modeling human listeriosis in natural and genetically engineered animals
Olivier Disson, Georgios Nikitas, Solène Grayo, Olivier Dussurget, Pascale Cossart & Marc Lecuit
Nature Protocols 4, 799 - 810 (2009) Published online: 7 May 2009
doi:10.1038/nprot.2009.66
Supplementary Figure 1: Principle of a competitive index assay
Supplementary Figure 1: Principle of a competitive index assay - Download PDF file (58KB)
Supplementary Video 1: Intravenous infection of an anesthetized gerbil with L. monocytogenes
(see manuscript for details)
Supplementary Video 2: Confocal 3D reconstruction of a gerbil placenta infected with EGD 42h pi
A 200 
m-thick section of a gerbil placenta was cut with a vibratome. The syncytiotrophoblast, placental capillaries and L. monocytogenes were stained in red (mouse anti-Ecad antibody), cyan (Isolectin B4) and green (rabbit anti-Listeria antibody), respectively. One hundred and fifty 200mx200m optical sections at 200 nm intervals were then imaged with a Leica TCS SP5 confocal microscope and a 3D-reconstruction was performed using the Imaris® software.
Supplementary Video 3: Confocal 3D reconstruction of a gerbil placenta infected with EGDΔinlAB 42h pi
A 200 m-thick section of a gerbil placenta was cut with a vibratome. The syncytiotrophoblast, placental capillaries and L. monocytogenes were stained in red (mouse anti-Ecad antibody), cyan (Isolectin B4) and green (rabbit anti-Listeria antibody), respectively. One hundred and fifty 250mx250m optical sections at 200 nm intervals were then imaged with a Leica TCS SP5 confocal microscope and a 3D-reconstruction was performed using the Imaris® software.
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