Volume 4 Issue 3, March 2009

Taking advantage of fluorophore-labeled oligonucleotides, the temperature-dependent assembly and disassembly of DNA nanostructures can be monitored in real time and with high throughput by FRET spectroscopy. Van't Hoff analysis of the fluorescence data provides insights into the thermodynamics of the self-assembly process. Image is from the protocol by Sacc[agrave] et al.