Protocol abstract
Nature Protocols 4, - 1737 - 1748 (2009)
Published online: 5 November 2009 | doi:10.1038/nprot.2009.185
Subject Categories: Model organisms | Imaging
Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia
Anne Limbourg1,2,3, Thomas Korff4, L Christian Napp1,3, Wolfgang Schaper5, Helmut Drexler3 & Florian P Limbourg1,3
Abstract
Blood vessel growth in adult organisms involves the following two fundamental processes: angiogenesis, the proliferation and extension of capillary networks; and arteriogenesis, the growth of functional arteries. We provide a protocol for the evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia. Surgical ligation of the femoral artery at a specific site triggers arteriogenesis of small, pre-existing collateral arteries into functional conduit vessels proximally and ischemic angiogenesis distally. The vascular response to hind-limb ischemia can be readily evaluated by laser Doppler-based perfusion measurements, histological quantification of arteriogenesis and angiogenesis or whole-mount visualization of arteries in limb muscles. Depending on the experimental design, the protocol takes between 4 and 29 d to complete; however, the net working time is about 2 d per mouse. The concurrent and specific analysis of postnatal angiogenesis and arteriogenesis in the same animal is a unique feature of the protocol.
- Junior Research Group Regenerative Agents, Excellence Cluster Rebirth, Hannover Medical School, Hannover, Germany.
- Integriertes Forschungs-und Behandlungszentrum (IFB-Tx), Hannover Medical School, Hannover, Germany.
- Department of Cardiology, Hannover Medical School, Hannover, Germany.
- Institute of Physiology and Pathophysiology, Division of Cardiovascular Physiology, University of Heidelberg, Heidelberg, Germany.
- Max–Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany.
Correspondence to: Anne Limbourg1,2,3 e-mail: limbourg.anne@mh-hannover.de
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A-Z product listing
- 1 Dumont Curved Tip Serrated forceps(Fine Science Tools)
- 2 Dumont Angled Tip Forceps(S&T JFAS))
- 25-G needle(BD Biosciences)
- 3-stop tubing(Ismatec)
- 4'-6-Diamidino-2-phenylindole(Sigma)
- 4'-6-Diamidino-2-phenylindole ((KMS Laborchemie Handels GMBH)
