Figure 2 - Structure of the microfabricated devices incorporating the ANA structure.

(a) shows the schematic of the ANA device, which includes the 5 mm 5 mm rectangular ANA, sample and buffer reservoirs, microfluidic channels surrounding the ANA. (b) shows a high-resolution photograph of the ANA device. (c,d) The scanning electron microscopy images of different regions of the ANA device (c: top view; d: cross-sectional or titled view). The planar ANA shown in c and d (top rows) have a period of 2 m along both the x and y directions (x direction: A'...A'; y direction: B'...B'), a width of 1.4 m (w_ps=1.4 m), a length of 1 m (l_ps=1 m) and a depth of 75 nm (d_ps=75 nm). Deep microfluidic channels are 1 m wide (w_pd) and 480 nm deep (d_pd). The vertical ANA shown in c and d (bottom rows) have a period of 3.5 m along both the x and y directions, a nanofilter gap width of 70 nm (w_vs=70 nm), and a wide region width of 1.2 m (w_vd=1.2 m). All the channels have the same depth of about 15 m. As indicated in a, after separation, the fractionated biomolecule streams are collected and routed to different fluid channels or reservoirs for convenient sample recovery and downstream bioanalysis.