Latest Protocols
Volume 4 No 1
- Archive
In order to view all protocols, including both Nature Protocols and Protocols Network protocols, please browse by category.
To facilitate archiving and citing, Nature Protocols are allocated to publication months and assigned unique page numbers.
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Nature protocols
Protein structure homology modeling using SWISS-MODEL workspace - pp1 - 13
Lorenza Bordoli, Florian Kiefer, Konstantin Arnold, Pascal Benkert, James Battey & Torsten Schwede
Published online: 11 December 2008 | doi:10.1038/nprot.2008.197
Abstract - Protein structure homology modeling using SWISS-MODEL workspace | Full Text - Protein structure homology modeling using SWISS-MODEL workspace | PDF (821 KB) - Protein structure homology modeling using SWISS-MODEL workspace
Subject Categories: Biochemistry and protein analysis | Computational and theoretical biology
Separation of sedimentary micron-sized particles for palaeoceanography and calcareous nannoplankton biogeochemistry - pp14 - 24
Fabrice Minoletti, Michaël Hermoso & Vincent Gressier
Published online: 11 December 2008 | doi:10.1038/nprot.2008.200
Abstract - Separation of sedimentary micron-sized particles for palaeoceanography and calcareous nannoplankton biogeochemistry | Full Text - Separation of sedimentary micron-sized particles for palaeoceanography and calcareous nannoplankton biogeochemistry | PDF (693 KB) - Separation of sedimentary micron-sized particles for palaeoceanography and calcareous nannoplankton biogeochemistry
Subject Category: Isolation purification and separation
Dictyostelium discoideum: a model host to measure bacterial virulence - pp25 - 30
Romain Froquet, Emmanuelle Lelong, Anna Marchetti & Pierre Cosson
Published online: 11 December 2008 | doi:10.1038/nprot.2008.212
Abstract - Dictyostelium discoideum: a model host to measure bacterial virulence | Full Text - Dictyostelium discoideum: a model host to measure bacterial virulence | PDF (218 KB) - Dictyostelium discoideum: a model host to measure bacterial virulence
Subject Categories: Microbiology and virology | Model organisms
Immunodesign of experimental sepsis by cecal ligation and puncture - pp31 - 36
Daniel Rittirsch, Markus S Huber-Lang, Michael A Flierl & Peter A Ward
Published online: 11 December 2008 | doi:10.1038/nprot.2008.214
Abstract - Immunodesign of experimental sepsis by cecal ligation and puncture | Full Text - Immunodesign of experimental sepsis by cecal ligation and puncture | PDF (339 KB) - Immunodesign of experimental sepsis by cecal ligation and puncture
Subject Categories: Microbiology and virology | Model organisms
Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues - pp37 - 43
Sylvia Streit, Christoph W Michalski, Mert Erkan, Jörg Kleeff & Helmut Friess
Published online: 11 December 2008 | doi:10.1038/nprot.2008.216
Abstract - Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues | Full Text - Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues | PDF (339 KB) - Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues
Subject Categories: Isolation purification and separation | Nucleic acid based molecular biology
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources - pp44 - 57
Da Wei Huang, Brad T Sherman & Richard A Lempicki
Published online: 18 December 2008 | doi:10.1038/nprot.2008.211
Abstract - Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources | Full Text - Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources | PDF (1,131 KB) - Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources | Supplementary information
Subject Category: Computational and theoretical biology
Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology - pp58 - 70
Pieter P Jacobs, Steven Geysens, Wouter Vervecken, Roland Contreras & Nico Callewaert
Published online: 18 December 2008 | doi:10.1038/nprot.2008.213
Abstract - Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology | Full Text - Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology | PDF (680 KB) - Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology
Subject Categories: Biochemistry and protein analysis | Microbiology and virology | Model organisms
Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment - pp71 - 77
Shoko Ueki, Benoît Lacroix, Alexander Krichevsky, Sondra G Lazarowitz & Vitaly Citovsky
Published online: 18 December 2008 | doi:10.1038/nprot.2008.217
Abstract - Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment | Full Text - Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment | PDF (323 KB) - Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment
Subject Categories: Genetic modification | Model organisms | Plant biology
Primary support cultures of hippocampal and substantia nigra neurons - pp78 - 85
Thomas Fath, Yazi D Ke, Peter Gunning, Jürgen Götz & Lars M Ittner
Published online: 18 December 2008 | doi:10.1038/nprot.2008.199
Abstract - Primary support cultures of hippocampal and substantia nigra neurons | Full Text - Primary support cultures of hippocampal and substantia nigra neurons | PDF (709 KB) - Primary support cultures of hippocampal and substantia nigra neurons
Subject Categories: Cell and tissue culture | Neuroscience
Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology - pp86 - 93
Alexander S Krupnick, Xue Lin, Wenjun Li, Mikio Okazaki, Jiaming Lai, Seiichiro Sugimoto, Steven B Richardson, Christopher G Kornfeld, Joel R Garbow, G Alexander Patterson, Andrew E Gelman & Daniel Kreisel
Published online: 18 December 2008 | doi:10.1038/nprot.2008.218
Abstract - Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology | Full Text - Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology | PDF (879 KB) - Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology
Subject Category: Model organisms
Assaying dynamic cell–cell junctional communication using noninvasive and quantitative fluorescence imaging techniques: LAMP and infrared-LAMP - pp94 - 101
Song Yang & Wen-Hong Li
Published online: 08 January 2009 | doi:10.1038/nprot.2008.219
Abstract - Assaying dynamic cell-cell junctional communication using noninvasive and quantitative fluorescence imaging techniques: LAMP and infrared-LAMP | Full Text - Assaying dynamic cell–cell junctional communication using noninvasive and quantitative fluorescence imaging techniques: LAMP and infrared-LAMP | PDF (443 KB) - Assaying dynamic cell–cell junctional communication using noninvasive and quantitative fluorescence imaging techniques: LAMP and infrared-LAMP | Supplementary information
Subject Categories: Imaging | Biochemistry and protein analysis
A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow - pp102 - 106
Masoud Soleimani & Samad Nadri
Published online: 08 January 2009 | doi:10.1038/nprot.2008.221
Abstract - A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow | Full Text - A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow | PDF (386 KB) - A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow
Subject Categories: Cell and tissue culture | Model organisms
A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets - pp107 - 115
Gerard J Nuovo, Terry S Elton, Patrick Nana-Sinkam, Stefano Volinia, Carlo M Croce & Thomas D Schmittgen
Published online: 08 January 2009 | doi:10.1038/nprot.2008.215
Abstract - A methodology for the combined : in situ: analyses of the precursor and mature forms of microRNAs and correlation with their putative targets | Full Text - A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets | PDF (762 KB) - A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets
Subject Category: Nucleic acid based molecular biology
